Plant Physiology Preview
Published on January 11, 2006; 10.1104/pp.105.072132
OPEN ACCESS ARTICLE
Received October 4, 2005
Returned for revision October 27, 2005
Accepted November 23, 2005
Nitrogen Fixation Mutants of Medicago truncatula Fail to Support Plant and Bacterial Symbiotic Gene Expression
Colby G. Starker , Adriana L. Parra-Colmenares , Lucinda Smith , Raka M. Mitra , and Sharon R. Long *
Department of Biological Sciences, Stanford University, Stanford, CA, 94305
* Corresponding author; email: srl{at}stanford.edu.
The Rhizobium-legume symbiosis culminates in the exchange of nutrients in the root nodule. Bacteria within the nodule reduce molecular nitrogen for plant use, and plants provide bacteria with carbon-containing compounds. Following the initial signaling events that lead to plant infection, little is known about the plant requirements for establishment and maintenance of the symbiosis. We screened 44,000 M2 plants from fast neutron irradiated Medicago truncatula seeds and isolated eight independent mutant lines that are defective in nitrogen fixation (DNF). The eight mutants are monogenic and represent seven complementation groups. To monitor bacterial status in mutant nodules, we assayed Sinorhizobium meliloti symbiosis gene promoters (nodF, exoY, bacA, and nifH) in the dnf mutants. Additionally, we used an Affymetrix oligonucleotide microarray to monitor gene expression changes in wild type and three mutant plants during the nodulation process. These analyses suggest the mutants can be separated into three classes: one class that supports little to no nitrogen fixation and minimal bacterial expression of nifH; another class supports no nitrogen fixation and minimal bacterial expression of exoY, bacA, and nifH; the final class supports low levels of both nitrogen fixation and bacterial nifH expression.
This article has been cited by other articles:
|
|
|
|
 
C. Pucciariello, G. Innocenti, W. Van de Velde, A. Lambert, J. Hopkins, M. Clement, M. Ponchet, N. Pauly, S. Goormachtig, M. Holsters, et al.
(Homo)glutathione Depletion Modulates Host Gene Expression during the Symbiotic Interaction between Medicago truncatula and Sinorhizobium meliloti
Plant Physiology,
November 1, 2009;
151(3):
1186 - 1196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Hernandez, O. Valdes-Lopez, M. Ramirez, N. Goffard, G. Weiller, R. Aparicio-Fabre, S. I. Fuentes, A. Erban, J. Kopka, M. K. Udvardi, et al.
Global Changes in the Transcript and Metabolic Profiles during Symbiotic Nitrogen Fixation in Phosphorus-Stressed Common Bean Plants
Plant Physiology,
November 1, 2009;
151(3):
1221 - 1238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Chen, C. Fan, M. Gao, and H. Zhu
Antiquity and Function of CASTOR and POLLUX, the Twin Ion Channel-Encoding Genes Key to the Evolution of Root Symbioses in Plants
Plant Physiology,
January 1, 2009;
149(1):
306 - 317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Vernie, S. Moreau, F. de Billy, J. Plet, J.-P. Combier, C. Rogers, G. Oldroyd, F. Frugier, A. Niebel, and P. Gamas
EFD Is an ERF Transcription Factor Involved in the Control of Nodule Number and Differentiation in Medicago truncatula
PLANT CELL,
October 1, 2008;
20(10):
2696 - 2713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Capoen, J. Den Herder, S. Rombauts, J. De Gussem, A. De Keyser, M. Holsters, and S. Goormachtig
Comparative Transcriptome Analysis Reveals Common and Specific Tags for Root Hair and Crack-Entry Invasion in Sesbania rostrata
Plant Physiology,
August 1, 2007;
144(4):
1878 - 1889.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Udvardi, K. Kakar, M. Wandrey, O. Montanari, J. Murray, A. Andriankaja, J.-Y. Zhang, V. Benedito, J. M.I. Hofer, F. Chueng, et al.
Legume Transcription Factors: Global Regulators of Plant Development and Response to the Environment
Plant Physiology,
June 1, 2007;
144(2):
538 - 549.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. I. Pislariu and R. Dickstein
An IRE-Like AGC Kinase Gene, MtIRE, Has Unique Expression in the Invasion Zone of Developing Root Nodules in Medicago truncatula
Plant Physiology,
June 1, 2007;
144(2):
682 - 694.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. H. Middleton, J. Jakab, R. V. Penmetsa, C. G. Starker, J. Doll, P. Kalo, R. Prabhu, J. F. Marsh, R. M. Mitra, A. Kereszt, et al.
An ERF Transcription Factor in Medicago truncatula That Is Essential for Nod Factor Signal Transduction
PLANT CELL,
April 1, 2007;
19(4):
1221 - 1234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
