Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 18, 2005; 10.1104/pp.105.072306

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
139/4/1935    most recent
pp.105.072306v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (22)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhu, S.
Right arrow Articles by Li, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhu, S.
Right arrow Articles by Li, Y.
Agricola
Right arrow Articles by Zhu, S.
Right arrow Articles by Li, Y.

Received October 6, 2005
Returned for revision October 6, 2005
Accepted October 19, 2005

The Rice Dwarf Virus P2 Protein Interacts with ent-Kaurene Oxidases in Vivo, Leading to Reduced Biosynthesis of Gibberellins and Rice Dwarf Symptoms

Shifeng Zhu , Feng Gao , Xuesong Cao , Mao Chen , Gongyin Ye , Chunhong Wei , and Yi Li *

Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, Beijing 100871, China

* Corresponding author; email: liyi{at}pku.edu.cn.

The mechanisms of viral diseases are a major focus of biology. Despite intensive investigations, how a plant virus interacts with host factors to cause diseases remains poorly understood. The Rice dwarf virus (RDV), a member of the genus Phytoreovirus, causes dwarfed growth phenotypes in infected rice (Oryza sativa) plants. The outer capsid protein P2 is essential during RDV infection of insects and thus influences transmission of RDV by the insect vector. However, its role during RDV infection within the rice host is unknown. By yeast two-hybrid and coimmunoprecipitation assays, we report that P2 of RDV interacts with ent-kaurene oxidases, which play a key role in the biosynthesis of plant growth hormones gibberellins, in infected plants. Furthermore, the expression of ent-kaurene oxidases was reduced in the infected plants. The level of endogenous GA1 (a major active gibberellin in rice vegetative tissues) in the RDV-infected plants was lower than that in healthy plants. Exogenous application of GA3 to RDV-infected rice plants restored the normal growth phenotypes. These results provide evidence that the P2 protein of RDV interferes with the function of a cellular factor, through direct physical interactions, that is important for the biosynthesis of a growth hormone leading to symptom expression. In addition, the interaction between P2 and rice ent-kaurene oxidase-like proteins may decrease phytoalexin biosynthesis and make plants more competent for virus replication. Moreover, P2 may provide a novel tool to investigate the regulation of GA metabolism for plant growth and development.




This article has been cited by other articles:


Home page
 Mol PlantHome page
D.-L. Yang, Q. Li, Y.-W. Deng, Y.-G. Lou, M.-Y. Wang, G.-X. Zhou, Y.-Y. Zhang, and Z.-H. He
Altered Disease Development in the eui Mutants and Eui Overexpressors Indicates that Gibberellins Negatively Regulate Rice Basal Disease Resistance
Mol Plant, May 13, 2008; (2008) ssn021v2.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M. Babu, A. G. Gagarinova, J. E. Brandle, and A. Wang
Association of the transcriptional response of soybean plants with soybean mosaic virus systemic infection
J. Gen. Virol., April 1, 2008; 89(4): 1069 - 1080.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Zhou, Y. Pu, T. Wei, H. Liu, W. Deng, C. Wei, B. Ding, T. Omura, and Y. Li
The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells
PNAS, December 4, 2007; 104(49): 19547 - 19552.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2005 by the American Society of Plant Biologists