Plant Physiology Preview
Published on January 27, 2006; 10.1104/pp.105.073825
Received November 7, 2005
Returned for revision December 20, 2005
Accepted December 26, 2005
Characterization of low phosphorus insensitive (lpi) mutants reveals a crosstalk between low P-induced determinate root development and the activation of genes involved in the adaptation of Arabidopsis to P deficiency
Lenin Sánchez-Calderón , José López-Bucio , Alejandra Chacón-López , Abel Gutiérrez-Ortega , Esmeralda Hernández-Abreu , and Luis Herrera-Estrella *
Departamento de Ingeniería Genética, Centro de Investigación y Estudios Avanzados, Cinvestav-Guanajuato, Apartado Postal 629, 36500 Irapuato, Guanajuato, México
Laboratorio Nacional de Genómica, Centro de Investigación y Estudios Avanzados, Cinvestav-Guanajuato, Apartado Postal 629, 36500 Irapuato, Guanajuato, México
* Corresponding author; email: lherrera{at}ira.cinvestav.mx.
Low phosphorus (P) availability is one of the most limiting factors for plant productivity in many natural and agricultural ecosystems. Plants display a wide range of adaptive responses to cope with low P stress, which generally serve to enhance P availability in the soil and to increase its uptake by roots. In Arabidopsis thaliana, primary root growth inhibition and increased lateral root formation have been reported to occur in response to P limitation. To gain knowledge of the genetic mechanisms that regulate root architectural responses to P availability, we designed a screen for identifying A. thaliana mutants that fail to arrest primary root growth when grown under low P conditions. Eleven low phosphorus insensitive (lpi) mutants that define at least four different complementation groups involved in primary root growth responses to P availability were identified. The lpi mutants do not show the typical determinate developmental program induced by P stress in the primary root. Other root developmental aspects of the low P rescue system including increased root hair elongation and anthocyanin accumulation remained unaltered in lpi mutants. In addition to the insensitivity of primary root growth inhibition, when subjected to P-deprivation, lpi mutants show a reduced induction in the expression of several genes involved in the P-starvation rescue system (AtPT1, AtPT2, PAP1, AtCP5 and AtIPS1). Our results provide genetic support for the role of P as an important signal for post-embryonic root development and root meristem maintenance and show a crosstalk in developmental and biochemical responses to P deprivation.
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