Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on January 11, 2006; 10.1104/pp.105.073858

OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrowOA All Versions of this Article:
140/2/624    most recent
pp.105.073858v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wang, B.-B.
Right arrow Articles by Brendel, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, B.-B.
Right arrow Articles by Brendel, V.
Agricola
Right arrow Articles by Wang, B.-B.
Right arrow Articles by Brendel, V.

Received November 7, 2005
Returned for revision November 26, 2005
Accepted November 26, 2005

Molecular characterization and phylogeny of U2AF35 homologs in plants

Bing-Bing Wang and Volker Brendel *

Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50010
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50010; Department of Statistics, Iowa State University, Ames, IA 50010

* Corresponding author; email: vbrendel{at}iastate.edu.

U2AF is an essential splicing factor with critical roles in recognition of the 3'-splice site. In animals, the U2AF small subunit (U2AF35) can bind to the 3'-AG intron border and promote U2 snRNP binding to the branchpoint sequences of introns through interaction with the U2AF large subunit. Two copies of U2AF35-encoding genes were identified in Arabidopsis (atU2AF35a and atU2AF35b). Both are expressed in all tissues inspected, with atU2AF35a expressed at a higher level than atU2AF35b in most tissues. Differences in the expression patterns of atU2AF35a and atU2AF35b in roots were revealed by a promoter::GUS assay, with atU2AF35b expressed strongly in whole young roots and root tips and atU2AF35a limited to root vascular regions. Altered expression levels of atU2AF35a or atU2AF35b cause pleiotropic phenotypes (including flowering time, leaf morphology, and flower and silique shape). Novel slicing isoforms were generated from FCA pre-mRNA by splicing of non-canonical introns in plants with altered expression levels of atU2AF35. U2AF35 homologs were also identified from maize, rice and other plants with large-scale EST projects. A C-terminal motif (named SERE) is highly conserved in all seed plant protein homologs, suggesting it may have an important function specific to higher plants.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Y. H. Jang, H.-Y. Park, S.-K. Kim, J. H. Lee, M. C. Suh, Y. S. Chung, K.-H. Paek, and J.-K. Kim
Survey of Rice Proteins Interacting With OsFCA and OsFY Proteins Which Are Homologous to the Arabidopsis Flowering Time Proteins, FCA and FY
Plant Cell Physiol., August 1, 2009; 50(8): 1479 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
W. B. Barbazuk, Y. Fu, and K. M. McGinnis
Genome-wide analyses of alternative splicing in plants: Opportunities and challenges
Genome Res., September 1, 2008; 18(9): 1381 - 1392.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists