Plant Physiology Preview
Published on January 13, 2006; 10.1104/pp.105.074385
Received November 18, 2005
Returned for revision December 9, 2005
Accepted January 9, 2006
High-affinity nitrate transport in roots of Arabidopsis thaliana depends on expression of the NAR2-like gene AtNRT3.1
Mamoru Okamoto , Anshuman Kumar , Wenbin Li , Ye Wang , M. Yaeesh Siddiqi , Nigel M. Crawford , and Anthony D.M. Glass *
Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116
Department of Botany, University of British Columbia, Vancouver, Canada V6T 1Z4
* Corresponding author; email: aglass{at}interchange.ubc.ca.
The NAR2 protein of Chlamydomonas has no known transport activity yet it is required for high-affinity nitrate uptake. Arabidopsis thaliana possesses two genes AtNRT3.1 and AtNRT3.2 that are similar to the Chlamydomonas NAR2 gene. AtNRT3.1 accounts for greater than 99% of NRT3 mRNA and is induced 6-fold by nitrate. AtNRT3.2 was expressed constitutively at a very low level and did not compensate for the loss of AtNRT3.1 in two Atnrt3.1 mutants. Nitrate uptake by roots and nitrate induction of gene expression were analyzed in two T-DNA mutants Atnrt3.1-1 and Atnrt3.1-2 disrupted in the AtNRT3.1 promoter and coding regions, respectively, in 5-week old plants. Nitrate induction of the nitrate transporter genes AtNRT1.1 and AtNRT2.1 was reduced in Atnrt3.1 mutant plants, and this reduced expression was correlated with reduced nitrate concentrations in the tissues. Constitutive high-affinity influx (CHATS) was reduced by 34% and by 89%, respectively in Atnrt3.1-1 and Atnrt3.1-2 mutant plants, while nitrate-inducible influx (IHATS) was reduced by 92% and 96%, respectively, following induction with 1 mM KNO3 after 7d of nitrogen deprivation. By contrast, low-affinity (LATS) influx appeared to be unaffected. These results provide the first in planta evidence that the CHATS and IHATS (but not LATS) of higher plant roots require a functional AtNRT3 (NAR2) gene.
This article has been cited by other articles:
|
|
|
|
 
S. Kant, Y.-M. Bi, E. Weretilnyk, S. Barak, and S. J. Rothstein
The Arabidopsis Halophytic Relative Thellungiella halophila Tolerates Nitrogen-Limiting Conditions by Maintaining Growth, Nitrogen Uptake, and Assimilation
Plant Physiology,
July 1, 2008;
147(3):
1168 - 1180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Fernandez and A. Galvan
Nitrate Assimilation in Chlamydomonas
Eukaryot. Cell,
April 1, 2008;
7(4):
555 - 559.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana
J. Biol. Chem.,
August 10, 2007;
282(32):
23541 - 23552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Miller, X. Fan, M. Orsel, S. J. Smith, and D. M. Wells
Nitrate transport and signalling
J. Exp. Bot.,
July 1, 2007;
58(9):
2297 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Fernandez and A. Galvan
Inorganic nitrogen assimilation in Chlamydomonas
J. Exp. Bot.,
July 1, 2007;
58(9):
2279 - 2287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Chopin, M. Orsel, M.-F. Dorbe, F. Chardon, H.-N. Truong, A. J. Miller, A. Krapp, and F. Daniel-Vedele
The Arabidopsis ATNRT2.7 Nitrate Transporter Controls Nitrate Content in Seeds
PLANT CELL,
May 1, 2007;
19(5):
1590 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tsujimoto, H. Yamazaki, S.-i. Maeda, and T. Omata
Distinct Roles of Nitrate and Nitrite in Regulation of Expression of the Nitrate Transport Genes in the Moss Physcomitrella patens
Plant Cell Physiol.,
March 1, 2007;
48(3):
484 - 497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Krouk, P. Tillard, and A. Gojon
Regulation of the High-Affinity NO3- Uptake System by NRT1.1-Mediated NO3- Demand Signaling in Arabidopsis
Plant Physiology,
November 1, 2006;
142(3):
1075 - 1086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Orsel, F. Chopin, O. Leleu, S. J. Smith, A. Krapp, F. Daniel-Vedele, and A. J. Miller
Characterization of a Two-Component High-Affinity Nitrate Uptake System in Arabidopsis. Physiology and Protein-Protein Interaction
Plant Physiology,
November 1, 2006;
142(3):
1304 - 1317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kawachi, Y. Sunaga, M. Ebato, T. Hatanaka, and H. Harada
Repression of Nitrate Uptake by Replacement of Asp105 by Asparagine in AtNRT3.1 in Arabidopsis thaliana L.
Plant Cell Physiol.,
October 1, 2006;
47(10):
1437 - 1441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
