Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 24, 2006; 10.1104/pp.105.074765

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
141/2/508    most recent
pp.105.074765v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakuragi, Y.
Right arrow Articles by Bryant, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakuragi, Y.
Right arrow Articles by Bryant, D. A.
Agricola
Right arrow Articles by Sakuragi, Y.
Right arrow Articles by Bryant, D. A.

Received January 1, 2006
Returned for revision January 30, 2006
Accepted March 10, 2006

{alpha}-Tocopherol plays a role in photosynthesis and macronutrient homeostasis of the cyanobacterium Synechocystis sp. PCC 6803 that is independent of its antioxidant function

Yumiko Sakuragi , Hiroshi Maeda , Dean DellaPenna , and Donald A. Bryant *

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824-1319; Cell and Molecular Biology Program, Michigan State University, East Lansing, MI, 48824-1319; DOE-Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824-1319
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824-1319; Cell and Molecular Biology Program, Michigan State University, East Lansing, MI, 48824-1319

* Corresponding author; email: dab14{at}psu.edu.

{alpha}-Tocopherol is synthesized exclusively in oxygenic phototrophs and is known to function as a lipid-soluble antioxidant. Here we report that {alpha}-tocopherol also has a novel function independent of its antioxidant properties in the cyanobacterium Synechocystis sp. PCC 6803. The photoautotrophic growth rates of wild type and mutants impaired in {alpha}-tocopherol biosynthesis are identical, but the mutants exhibit elevated photosynthetic activities and glycogen levels. When grown photomixotrophically with glucose, however, these mutants cease growth within 24 h and exhibit a global macronutrient starvation response associated with nitrogen, sulfur, and carbon, as shown by decreased phycobiliprotein content (35% of the wild-type level) and accumulation of the nblA1-nblA2, sbpA, sigB, sigE, and sigH transcripts. PS II activity and carboxysome synthesis are lost in the tocopherol mutants within 24 h of photomixotrophic growth, and the abundance of carboxysome gene (rbcL, ccmK1, ccmL) and ndhF4 transcripts decreases to undetectable levels. These results suggest that {alpha}-tocopherol plays an important role in optimizing photosynthetic activity and macronutrient homeostasis in Synechocystis sp. PCC 6803. Several lines of evidence indicate increased oxidative stress in the tocopherol mutants is unlikely to be the underlying cause of PS II inactivation and glucose-induced lethality. Interestingly, insertional inactivation of the pmgA gene, which encodes a putative serine/threonine kinase similar to RsbW and RsbT in Bacillus subtilis, results in a similar increase in glycogen and glucose-induced lethality. Based on these results, we propose that {alpha}-tocopherol plays a non-antioxidant regulatory role in photosynthesis and macronutrient homeostasis through a signal transduction pathway that also involves PmgA.




This article has been cited by other articles:


Home page
 J Exp BotHome page
H. Takahashi, H. Uchimiya, and Y. Hihara
Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry
J. Exp. Bot., August 1, 2008; 59(11): 3009 - 3018.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Y. Yang, C. Yin, W. Li, and X. Xu
{alpha}-Tocopherol Is Essential for Acquired Chill-Light Tolerance in the Cyanobacterium Synechocystis sp. Strain PCC 6803
J. Bacteriol., March 1, 2008; 190(5): 1554 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Maeda, T. L. Sage, G. Isaac, R. Welti, and D. DellaPenna
Tocopherols Modulate Extraplastidic Polyunsaturated Fatty Acid Metabolism in Arabidopsis at Low Temperature
PLANT CELL, February 1, 2008; 20(2): 452 - 470.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Lee, J.-Y. Ryu, S. Y. Kim, J.-H. Jeon, J. Y. Song, H.-T. Cho, S.-B. Choi, D. Choi, N. T. de Marsac, and Y.-I. Park
Transcriptional Regulation of the Respiratory Genes in the Cyanobacterium Synechocystis sp. PCC 6803 during the Early Response to Glucose Feeding
Plant Physiology, November 1, 2007; 145(3): 1018 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A.-R. Abbasi, M. Hajirezaei, D. Hofius, U. Sonnewald, and L. M. Voll
Specific Roles of {alpha}- and {gamma}-Tocopherol in Abiotic Stress Responses of Transgenic Tobacco
Plant Physiology, April 1, 2007; 143(4): 1720 - 1738.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. E. Sattler, L. Mene-Saffrane, E. E. Farmer, M. Krischke, M. J. Mueller, and D. DellaPenna
Nonenzymatic Lipid Peroxidation Reprograms Gene Expression and Activates Defense Markers in Arabidopsis Tocopherol-Deficient Mutants
PLANT CELL, December 1, 2006; 18(12): 3706 - 3720.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists