Plant Physiology Preview
Published on February 24, 2006; 10.1104/pp.105.074898
Received November 29, 2005
Returned for revision January 6, 2006
Accepted January 26, 2006
Arabidopsis Hsa32, a novel heat-shock protein, is essential for acquired thermotolerance during long recovery after acclimation
Yee-yung Charng *, Hsiang-chin Liu , Nai-yu Liu , Fu-chiun Hsu , and Swee-suak Ko
Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan 11529, ROC
* Corresponding author; email: yycharng{at}gate.sinica.edu.tw.
Plants and animals share similar mechanisms in the heat-shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. Here, we report on the analysis of the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss, Arabidopsis, and rice. Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (> 24 h) after acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Quantitative hypocotyl elongation assay also revealed that thermotolerance decayed faster in the absence of Hsa32 after a long recovery. Similar results were obtained in Arabidopsis transgenic plants with Hsa32 expression suppressed by RNAi. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is required not for induction but, rather, maintenance of acquired thermotolerance, a feature that could be important to plants.
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