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Published on February 3, 2006; 10.1104/pp.105.074922

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Received November 30, 2005
Returned for revision December 28, 2005
Accepted January 19, 2006

LlANK, Characterized as a Ubiquitin Ligase, Is Closely Associated with Membrane-enclosed Organelles and Required for Pollen Germination and Pollen Tube Growth in Lilium longiflorum

Jian Huang , Feng Chen , Cecilia Del Casino , Antonella Autino , Mouhua Shen , Shuai Yuan , Jia Peng , Hexin Shi , Chen Wang , Mauro Cresti , and Yiqin Li *

Protein Science Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnologies, Tsinghua University, Beijing 100084, China
Dipartimento di Scienze Ambientali "G. Sarfatti", Università di Siena, Via P.A. Mattioli, 4-53100 Siena Italia
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

* Corresponding author; email: liyq{at}mail.tsinghua.edu.cn.

Exhibiting rapid polarized growth, pollen tube delivers the male gametes into ovule for fertilization in higher plants. To get an overall picture of gene expression during pollen germination and pollen tube growth, we profiled the transcription patterns of 1536 pollen cDNAs from Lilium longiflorum by microarray. Among those that exhibited significant differential expression, a cDNA named LlANK (L. longiflorum ankyrin repeat- containing protein) was thoroughly studied. The full-length LlANK cDNA sequence predicts a protein containing five tandem ankyrin repeats and a RING Zn-finger domain. The LlANK protein possesses ubiquitin ligase activity in vitro. RNA blots demonstrated that LlANK transcript is present in mature pollen and its level, interestingly contrary to most pollen mRNAs, up-regulated significantly during pollen germination and pollen tube growth. When fused with green fluorescent protein and transiently expressed in pollen, LlANK was found dominantly associated with membrane-enclosed organelles as well as the generative cell. Overexpression of LlANK, however, led to abnormal growth of pollen tube. On the other hand, transient silencing of LlANK impaired pollen germination and tube growth. Taken together, these results showed that LlANK is a ubiquitin ligase associated with membrane-enclosed organelles and required for polarized pollen tube growth.




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[Abstract] [Full Text] [PDF]


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