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Published on January 13, 2006; 10.1104/pp.105.075838

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Received December 19, 2005
Returned for revision December 23, 2005
Accepted December 23, 2005

Interaction between Arabidopsis Brca2 and its partners: Rad51, Dmc1 and Dss1

Eloïse Dray , Nicolas Siaud , Emeline Dubois , and Marie-Pascale Doutriaux *

Institut de Biotechnologie des Plantes, UMR CNRS 8618, Bâtiment 630, Université Paris XI, 91405 Orsay cedex, France
Institut de Biotechnologie des Plantes, UMR CNRS 8618, Bâtiment 630, Université Paris XI, 91405 Orsay cedex, France; Present address : Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021, USA

* Corresponding author; email: doutriau{at}ibp.u-psud.fr.

The Arabidopsis orthologs of Brca2, a protein whose mutations are involved in breast cancer in human, were previously shown to be essential at meiosis. In an attempt to better understand the Brca2 interacting properties, we examined four partners of the two isoforms of Brca2 identified in Arabidopsis thaliana (AtRad51, AtDmc1, and two AtDss1 isoforms). The two Brca2 and the two Dss1 isoforms are named AtBrca2(IV) and AtBrca2(V) and AtDss1(I) and AtDss1(V) after their chromosomal localization. We first show that both AtBrca2 proteins can interact with either AtRad51 or AtDmc1 in vitro and that the N-terminal region of AtBrca2 is responsible for these interactions. More specifically, the BRC motifs in Brca2 are involved in these interactions: BRC motif number 2 (BRC2) alone can interact with AtDmc1 whereas BRC motif number 4 (BRC4) recognizes AtRad51. The human Rad51 and Dmc1 proteins themselves can interact with either the complete (HsRad51) or a shorter version of AtBrca2 (HsRad51, HsDmc1) that comprises all four BRC motifs. We also identified two Arabidopsis isoforms of Dss1, another known partner of Brca2 in other organisms. Although all four Brca2 and Dss1 proteins are very conserved, AtBrca2(IV) interacts with only one of these AtDss1 protein whereas AtBrca2(V) interacts whith both of them. Finally, we show for the first time that an AtBrca2 protein could bind two different partners at the same time : either AtRad51 and AtDss1(I) or AtDmc1 and AtDss1(I).






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