Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on February 17, 2006; 10.1104/pp.105.076059

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
140/4/1255    most recent
pp.105.076059v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pischke, M. S.
Right arrow Articles by Sussman, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pischke, M. S.
Right arrow Articles by Sussman, M. R.
Agricola
Right arrow Articles by Pischke, M. S.
Right arrow Articles by Sussman, M. R.

Received December 21, 2005
Returned for revision January 16, 2006
Accepted February 6, 2006

A transcriptome-based characterization of habituation in plant tissue culture

Melissa S. Pischke , Edward L. Huttlin , Adrian D. Hegeman , and Michael R. Sussman *

Department of Biochemistry, University of Wisconsin, Biotechnology Center, 425 Henry Mall, Madison, WI 53706

* Corresponding author; email: msussman{at}wisc.edu.

For the last 50 years, scientists have recognized that varying ratios of the plant hormones cytokinin and auxin induce plant cells to form particular tissues: undifferentiated callus, shoot structures, root structures, or a whole plant. Proliferation of undifferentiated callus tissue, greening, and the formation of shoot structures are all cytokinin-dependent processes. Habituation refers to a naturally occurring phenomenon whereby callus cultures, upon continued passage, lose their requirement for cytokinin. Earlier studies of calli derived from plants with a higher-than-normal cytokinin content, indicate that overproduction of cytokinin by the culture tissues is a possible explanation for this acquired cytokinin-independence. A transcriptome-based analysis of a well established habituated Arabidopsis thaliana cell culture line was undertaken, to explore genome-wide expression changes underlying the phenomenon of habituation. Altered levels of expression of the cytokinin receptor CRE1, as well as several other genes involved in cytokinin signaling, indicated that naturally-acquired deregulation of cytokinin-signaling components could play a previously unrecognized role in habituation. Up-regulation of several cytokinin oxidases, down-regulation of several known cytokinin-inducible genes, and a lack of regulation of the cytokinin synthases indicated that increases in hormone concentration may not be required for habituation. In addition up-regulation of, the homeodomain transcription factor FWA, transposon-related elements, and several DNA- and chromatin-modifying enzymes indicated that epigenetic changes contribute to the acquisition of cytokinin-habituation.




This article has been cited by other articles:


Home page
 J Exp BotHome page
W. Lin, Y. Peng, G. Li, R. Arora, Z. Tang, W. Su, and W. Cai
Isolation and functional characterization of PgTIP1, a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng
J. Exp. Bot., March 1, 2007; 58(5): 947 - 956.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists