Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 21, 2006; 10.1104/pp.105.076109

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
141/2/598    most recent
pp.105.076109v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jones, L.
Right arrow Articles by Vaistij, F. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jones, L.
Right arrow Articles by Vaistij, F. E.
Agricola
Right arrow Articles by Jones, L.
Right arrow Articles by Vaistij, F. E.

Received December 29, 2005
Returned for revision January 30, 2006
Accepted April 6, 2006

Virus-induced Gene Silencing of Argonaute genes in Nicotiana benthamiana demonstrates that extensive systemic silencing requires AGO1 and AGO4-like genes

Louise Jones *, Teresa Keining , Andrew Eamens , and Fabián E. Vaistij

Dept. of Biology, University of York, York, YO10 5YW, U.K
Centre for Novel Agricultural Products, University of York, York, YO10 5YW, U.K

* Corresponding author; email: alj2{at}york.ac.uk.

Several distinct pathways of RNA silencing operate in plants with roles including the suppression of virus accumulation, control of endogenous gene expression and direction of DNA and chromatin modifications. Proteins of the Dicer-Like (DCL) and Argonaute (AGO) families have key roles within these silencing pathways and have distinct biochemical properties. We are interested in the relationships between different silencing pathways and have used Nicotiana benthamiana as a model system. Whilst not being an amenable plant for traditional genetics, N. benthamiana is extensively used for RNA silencing studies. Using virus-induced gene silencing (VIGS) technology we demonstrate that both NbAGO1 and NbAGO4-like genes are required for full systemic silencing but not for silencing directed by an inverted repeat (IR) transgene. Phenotypic differences between the VIGS plants indicate that NbAGO1 and NbAGO4-like act at different stages of the silencing pathways. Suppression of NbAGO1 expression recapitulated the hypomorphic mutant phenotype of certain Arabidopsis thaliana ago1 alleles, however, suppression of NbAgo4-like resulted in phenotypes differing in some respects from those reported for Arabidopsis ago4. We suggest that the small interfering RNA (siRNA) amplification step required for full systemic silencing is dependent upon a nuclear event requiring the activity of NbAGO4-like.




This article has been cited by other articles:


Home page
 Mol PlantHome page
R. Grant-Downton, S. Hafidh, D. Twell, and H. G. Dickinson
Small RNA Pathways Are Present and Functional in the Angiosperm Male Gametophyte
Mol Plant, May 1, 2009; 2(3): 500 - 512.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. A. Brosnan, N. Mitter, M. Christie, N. A. Smith, P. M. Waterhouse, and B. J. Carroll
Nuclear gene silencing directs reception of long-distance mRNA silencing in Arabidopsis
PNAS, September 11, 2007; 104(37): 14741 - 14746.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists