Plant Physiology Preview
Published on April 7, 2006; 10.1104/pp.106.077123
Received January 13, 2006
Returned for revision February 17, 2006
Accepted March 31, 2006
Arabidopsis thaliana INOSITOL TRANSPORTER4 mediates high affinity H+-symport of myo-inositol across the plasma membrane
Sabine Schneider , Alexander Schneidereit , Kai R. Konrad , Mohammad-Reza Hajirezaei , Monika Gramann , Rainer Hedrich , and Norbert Sauer *
Molekulare Pflanzenphysiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, D-91058 Erlangen, Germany
Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Molekulare Pflanzenphysiologie und Biophysik, D-97082 Würzburg, Germany
Institut für Pflanzengenetik und Kulturpflanzenforschung, D-06466 Gatersleben, Germany
* Corresponding author; email: nsauer{at}biologie.uni-erlangen.de.
Four genes of the Arabidopsis monosaccharide transporter-like (MST-like) superfamily share significant homology with transporter genes previously identified in the common ice plant, Mesembryanthemum crystallinum, a model system for studies on salt toler-ance of higher plant. These ice plant transporters had been discussed as tono-plast proteins catalyzing the inositol-dependent efflux of Na+ ions from vacuoles. The sub-cellular localization and the physiological role of the homologous proteins in the glyco-phyte Arabidopsis were unclear. Here we describe AtINT4, the first member of this subgroup of Arabi-dopsis MST-like transporters. Functional analyses of the protein in Saccharomy-ces cerevisiae and Xenopus laevis oocytes characterize this protein as a highly spe-cific H+-symporter for myo-inositol. These activities and analyses of the subcellular local-ization of an AtINT4 fusion protein in Arabidopsis and tobacco reveal that AtINT4 is located in the plasma membrane. AtINT4 promoter-reporter gene plants demon-strate that AtINT4 is strongly expressed in Arabidopsis pollen and in phloem compan-ion cells. The potential physiological role of AtINT4 is discussed.
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