Plant Physiology Preview
Published on April 14, 2006; 10.1104/pp.106.077149
Received January 17, 2006
Returned for revision February 21, 2006
Accepted April 7, 2006
Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in Arabidopsis
Isabel Ochando , Sara Jover-Gil , Juan José Ripoll , Héctor Candela , Antonio Vera , María Rosa Ponce , Antonio Martínez-Laborda , and José Luis Micol *
División de Genética, Universidad Miguel Hernández, Campus de San Juan, 03550 Alicante, Spain
División de Genética and Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Alicante, Spain
* Corresponding author; email: jlmicol{at}umh.es.
Here, we describe how the semidominant, gain-of-function icu4-1 and icu4-2 alleles of the INCURVATA4 gene alter leaf phyllotaxis and cell organization in the root apical meristem, reduce root length, and cause xylem overgrowth in the stem. The ICU4 gene was positionally cloned and found to encode the ATHB15 transcription factor, a class III homeodomain/leucine zipper (HD-Zip III) family member, recently named CORONA. The icu4-1 and icu4-2 alleles bear the same point mutation that affects the microRNA complementarity site of ICU4 and is identical to those of several semidominant alleles of the HD-Zip III family members PHABULOSA and PHAVOLUTA. The icu4-1 and icu4-2 mutations significantly increase leaf transcript levels of the ICU4 gene. The null hst-1 allele of the HASTY gene, which encodes a nucleocytoplasmic transporter, synergistically interacts with icu4-1, the double mutant displaying partial adaxialization of rosette leaves and carpels. Our results suggest that the ICU4 gene has an adaxializing function and that it is downregulated by microRNAs that require the HST protein for their biogenesis.
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