Plant Physiology Preview
Published on March 2, 2006; 10.1104/pp.106.077990
Received January 27, 2006
Returned for revision February 11, 2006
Accepted February 14, 2006
INTERPLAY BETWEEN ARABIDOPSIS ACTIVATING FACTORS E2FB AND E2FA IN CELL CYCLE PROGRESSION AND DEVELOPMENT
Rosangela Sozzani , Caterina Maggio , Serena Varotto , Sabrina Canova , Catherine Bergounioux , Diego Albani , and Rino Cella *
Department of Genetics and Microbiology, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
Department of Environmental Agronomy and Crop Production, University of Padova, Agripolis, Via Romea 16, 35020 Legnaro, Italy
Institute of Plant Biotechnology, University of Paris-XI, 91400 Orsay, France
Department of Botany and Plant Ecology, University of Sassari, Via Muroni 25, 07100 Sassari, Italy
* Corresponding author; email: cella{at}ipvgen.unipv.it.
Eukaryotic E2Fs are conserved transcription factors playing crucial and antagonistic roles in several pathways related to cell division, DNA repair and differentiation. In plants these processes are strictly intermingled at the growing zone to produce post-embryonic development in response to internal signals and environmental cues. Of the six AtE2F proteins found in Arabidopsis thaliana only AtE2Fa and AtE2Fb have been clearly indicated as activators of E2F-responsive genes. AtE2Fa activity was shown to induce S phase and endoreduplication, whereas the function of AtE2Fb and the interrelationship between these two transcription factors was unclear. We have investigated the role played by the AtE2Fb gene during cell cycle and development performing in situ RNA hybridization, immunolocalization of the AtE2Fb protein in planta and analysis of the AtE2Fb promoter activity in transgenic plants. Overexpression of AtE2Fb in transgenic Arabidopsis plants led to striking modifications of the morphology of roots, cotyledons and leaves that can be ascribed to stimulation of cell division. The accumulation of the AtE2Fb protein in these lines was paralleled by an increased expression of E2F-responsive G1/S and G2/M marker genes. These results suggest that AtE2Fa and AtE2Fb have specific expression patterns and play similar but distinct roles during cell cycle progression.
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