Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on March 24, 2006; 10.1104/pp.106.078048

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
141/1/271    most recent
pp.106.078048v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sekimoto, H.
Right arrow Articles by Ito, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sekimoto, H.
Right arrow Articles by Ito, M.
Agricola
Right arrow Articles by Sekimoto, H.
Right arrow Articles by Ito, M.

Received January 28, 2006
Returned for revision February 23, 2006
Accepted March 11, 2006

Gene expression profiling using cDNA microarray analysis of the sexual reproduction stage of the unicellular charophycean alga Closterium peracerosum-strigosum-littorale complex

Hiroyuki Sekimoto *, Yoichi Tanabe , Yuki Tsuchikane , Hiroshi Shirosaki , Hiroo Fukuda , Taku Demura , and Motomi Ito

Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan
Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Meguro-ku, Tokyo 153-8902, Japan
Plant Science Center, RIKEN, Yokohama 230-0045, Japan; Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
Plant Science Center, RIKEN, Yokohama 230-0045, Japan

* Corresponding author; email: sekimoto{at}fc.jwu.ac.jp.

The desmid Closterium peracerosum-strigosum-littorale complex, which is the closest unicellular sister to land plants, is the best-characterized of the charophycean green algae with respect to the process of sexual reproduction. To elucidate the molecular mechanism of intercellular communication during sexual reproduction, we created a normalized cDNA library from mixed cells of the sexual and the vegetative phases, and generated a cDNA microarray. In total, 3,236 ESTs, which were classified into 1,615 nonredundant groups, were generated for cDNA microarray construction. Candidate genes for key factors involved in fertilization, such as those that encode putative receptor-like protein kinases, leucine-rich-repeat receptor-like proteins, and sex pheromone homologues, were up-regulated during sexual reproduction and/or by the addition of the purified sex pheromones, and the expression patterns of these genes were confirmed by quantitative real-time PCR analysis. This first transcriptome profile of Closterium will provide critical clues as to the mechanism and evolution of intercellular communication between the egg and sperm cells of land plants.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Kubo, J. Abe, T. Oyamada, M. Ohnishi, H. Fukuzawa, Y. Matsuda, and T. Saito
Characterization of novel genes induced by sexual adhesion and gamete fusion and of their transcriptional regulation in Chlamydomonas reinhardtii
Plant Cell Physiol., June 1, 2008; 49(6): 981 - 993.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Abe, Y. Hiwatashi, M. Ito, M. Hasebe, and H. Sekimoto
Expression of Exogenous Genes Under the Control of Endogenous HSP70 and CAB Promoters in the Closterium peracerosum-strigosum-littorale complex
Plant Cell Physiol., April 1, 2008; 49(4): 625 - 632.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists