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Published on May 5, 2006; 10.1104/pp.106.078626

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Received February 2, 2006
Returned for revision February 23, 2006
Accepted May 1, 2006

Molecular Cloning and Characterization of {beta}-Expansin Gene Related to Root Hair Formation in Barley

Miroslaw Kwasniewski * and Iwona Szarejko

Department of Genetics, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland

* Corresponding author; email: kwasniew{at}us.edu.pl.

Root hairs are specialized epidermal cells that play a role in uptake of water and nutrients from rhizosphere and serve as a site of interaction with soil microorganisms. Process of root hair formation is well characterized in Arabidopsis thaliana, however there is a very few information about genetic and molecular basis of root hair development in monocots. Here we report on isolation and cloning of the {beta}-expansin gene HvEXPB1, tightly related to root hair initiation in barley (Hordeum vulgare L.). Using root transcriptome differentiation in the wild type/root-hairless mutant system, a cDNA fragment present in roots of wild-type plants only was identified. After cloning of full length cDNA and genomic sequences flanking the identified fragment, the subsequent bioinformatics analyses revealed homology of the protein coded by the identified gene to the {beta}-expansin family. RT-PCR showed that the expression of HvEXPB1 co-segregated with root hair phenotype in F2 progeny of the cross between the hairless mutant rh1.a and wild-type parent variety ‘Karat’. The expression of HvEXPB1 gene was root-specific - it was expressed in roots of wild type forms but not in coleoptiles, leaves, tillers and spikes. The identified gene was active in roots of two other analyzed root hair mutants: rhp1.a developing root hair primordia only and rhs1.a with very short root hairs. Contrary to this, the complete lack of HvEXPB1 expression was observed in roots of the spontaneous, root-hairless mutant brb. All these observations suggest the role of HvEXPB1 gene in the process of root hair formation in barley.




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