Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 14, 2006; 10.1104/pp.106.078881

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
141/2/527    most recent
pp.106.078881v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, S.
Right arrow Articles by Rhee, S. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, S.
Right arrow Articles by Rhee, S. Y.
Agricola
Right arrow Articles by Li, S.
Right arrow Articles by Rhee, S. Y.

Received February 7, 2006
Returned for revision February 23, 2006
Accepted April 7, 2006

Systematic Analysis of Arabidopsis Organelles and a Protein Localization Database for Facilitating Fluorescent Tagging of Full-Length Arabidopsis Proteins

Shijun Li , David W. Ehrhardt , and Seung Y. Rhee *

Carnegie Institution, Department of Plant Biology, Stanford, California 94305

* Corresponding author; email: rhee{at}acoma.stanford.edu.

Cells are organized into a complex network of subcellular compartments that are specialized for various biological functions. Subcellular location is an important attribute of protein function. In order to facilitate systematic elucidation of protein subcellular location, we analyzed experimentally verified protein localization data of 1,300 Arabidopsis proteins. The 1,300 experimentally verified proteins are distributed among 40 different compartments, with most of the proteins localized to four compartments: mitochondria (36 %), nucleus (28 %), plastid (17%) and cytosol (13.3 %). About 19% of the proteins are found in multiple compartments, in which a high proportion (36.4%) is localized to both cytosol and nucleus. Characterization of the over-represented Gene Ontology molecular functions and biological processes suggests that the Golgi apparatus and peroxisome may play more diverse functions but are involved in more specialized processes than other compartments. To support systematic empirical determination of protein subcellular localization using a technology called fluorescent tagging of full-length proteins (FTFLP), we developed a database and web application to provide pre-selected green fluorescent protein (GFP) insertion position and primer sequences for all Arabidopsis proteins to study their subcellular localization and to store experimentally verified protein localization images, videos, and their annotations of proteins generated using the FTFLP technology. The database can be searched, browsed and downloaded using a web browser at http://aztec.stanford.edu/gfp/. The software can also be downloaded from the same website for local installation.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Q.-F. Chen, S. Xiao, and M.-L. Chye
Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance
Plant Physiology, September 1, 2008; 148(1): 304 - 315.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. M. Goodin, R. Chakrabarty, R. Banerjee, S. Yelton, and S. DeBolt
New Gateways to Discovery
Plant Physiology, December 1, 2007; 145(4): 1100 - 1109.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. L. Heazlewood, R. E. Verboom, J. Tonti-Filippini, I. Small, and A. H. Millar
SUBA: the Arabidopsis Subcellular Database
Nucleic Acids Res., January 12, 2007; 35(suppl_1): D213 - D218.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists