Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on May 26, 2006; 10.1104/pp.106.079046

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
141/3/1089    most recent
pp.106.079046v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cohen, I.
Right arrow Articles by Shapira, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cohen, I.
Right arrow Articles by Shapira, M.
Agricola
Right arrow Articles by Cohen, I.
Right arrow Articles by Shapira, M.

Received February 9, 2006
Returned for revision March 23, 2006
Accepted May 4, 2006

A conserved mechanism controls translation of Rubisco large subunit in different photosynthetic organisms

Idan Cohen , Yair Sapir , and Michal Shapira *

Department of Life Sciences, Ben-Gurion University of the Negev, POBox 653, Beer-Sheva 84105, Israel

* Corresponding author; email: shapiram{at}bgu.ac.il.

We previously proposed a novel mechanism for control of ribulose-1,5 biphosphate carboxylase/oxygenase (Rubisco) expression and assembly during oxidative stress in Chlamydomonas reinhardtii. The N-terminus of the large subunit (LSU) comprises an RNA recognition motif (RRM) that is normally buried in the protein, but becomes exposed under oxidizing conditions, when the glutathione pool shifts towards its oxidized form. Thus, de-novo translation and assembly of Rubisco LSU stop with similar kinetics, and the unpaired small subunit (SSU) is rapidly degraded. Here we show that the structure of the N-terminal domain is highly conserved throughout evolution, despite its relatively low sequence similarity. Furthermore, Rubisco from a broad evolutionary range of photosynthetic organisms binds RNA under oxidizing conditions, with Kd values in the nano-molar range. In line with these observations, oxidative stress indeed causes a translational arrest in land plants as well as in Rhodospirillum rubrum, a purple bacterium that lacks the SSU. We highlight an evolutionary conserved element located within {alpha}-helix B, which is located in the center of the RRM, and is also involved in the intramolecular interactions between two LSU chains. Thus, assembly masks the N-terminus of the LSU hiding the RRM. When assembly is interrupted due to structural changes that occur under oxidizing conditions, or in the absence of a dedicated chaperone, the N-terminal domain can become exposed, leading to the translational arrest of Rubisco LSU. Taken together, these results support a model by which LSU translation is governed by its dimerization. In case that regulation of type I and type II Rubisco is conserved, the SSU does not appear to be directly involved in LSU translation.




This article has been cited by other articles:


Home page
 JCBHome page
J. Uniacke and W. Zerges
Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii
J. Cell Biol., August 25, 2008; 182(4): 641 - 646.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Ichikawa, C. Miyake, M. Iwano, M. Sekine, A. Shinmyo, and K. Kato
Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Translation is Regulated in a Small Subunit-Independent Manner in the Expanded Leaves of Tobacco
Plant Cell Physiol., February 1, 2008; 49(2): 214 - 225.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Wostrikoff and D. Stern
Rubisco large-subunit translation is autoregulated in response to its assembly state in tobacco chloroplasts
PNAS, April 10, 2007; 104(15): 6466 - 6471.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists