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Published on June 15, 2006; 10.1104/pp.106.079277

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Received February 14, 2006
Returned for revision March 28, 2006
Accepted June 5, 2006

Molecular changes occurring during acquisition of abscission competence following auxin depletion in Mirabilis jalapa L

Shimon Meir *, Donald A. Hunter , Jen-Chih Chen , Vita Halaly , and Michael S. Reid

Department of Postharvest Science of Fresh Produce, ARO, The Volcani Center, Bet Dagan 50250, Israel
New Zealand Institute for Crop & Food Research Limited, Private Bag 11600, Palmerston North, New Zealand
Department of Plant Sciences, University of California, Davis, CA 95616, USA

* Corresponding author; email: shimonm{at}volcani.agri.gov.il.

To understand how auxin regulates sensitivity of abscission zone (AZ) tissues to ethylene, we used a PCR-based subtractive approach to identify gene transcripts in Mirabilis jalapa L. AZs that changed in abundance during the time the zones became competent to abscise in response to exogenous ethylene. Transcript expression was then examined in leaf and stem AZs over the period they became ethylene competent following indole-3-acetic acid (IAA) depletion either by leaf deblading, treatment with the IAA transport inhibitor naphthylphthalamic acid, or cutting the stem above a node (decapitation). Transcripts down-regulated by deblading/decapitation, included Mj-Aux/IAA1 and Mj-Aux/IAA2, encoding Aux/IAA proteins, and three other transcripts showing highest identity to a polygalacturonase inhibitor protein, a {beta}-expansin and a {beta}-tubulin. Application of IAA to the cut end of petioles or stumps inhibited abscission, and prevented the decline in the levels of transcripts in both AZs. Transcripts up-regulated in the AZ following deblading/decapitation or treatment with naphthylphthalamic acid, were isolated from plants pretreated with 1-methylcyclopropene before deblading to help select against ethylene-induced genes. Some of the up-regulated transcripts showed identity to proteins associated with ethylene or stress responses, while others did not show homology to known sequences. Sucrose infiltration of stem stumps enhanced abscission following ethylene treatment and also enhanced the induction of some of the up-regulated genes. Our results demonstrate, for the first time, a correlation between acquisition of competence to respond to ethylene in both leaf and stem AZs, and decline in abundance of auxin regulatory gene transcripts.




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B. Abebie, A. Lers, S. Philosoph-Hadas, R. Goren, J. Riov, and S. Meir
Differential Effects of NAA and 2,4-D in Reducing Floret Abscission in Cestrum (Cestrum elegans) Cut Flowers are Associated with their Differential Activation of Aux/IAA Homologous Genes
Ann. Bot., January 1, 2008; 101(2): 249 - 259.
[Abstract] [Full Text] [PDF]


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