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Published on May 19, 2006; 10.1104/pp.106.079384

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Received February 17, 2006
Returned for revision March 20, 2006
Accepted April 30, 2006

QTL analysis of primary cell wall composition in Arabidopsis thaliana

Grégory Mouille , Hanna Witucka-Wall , Marie-Pierre Bruyant , Olivier Loudet , Christophe Rihouey , Olivier Lerouxel , Patrice Lerouge , Herman Höfte *, and Markus Pauly

Laboratoire de Biologie Cellulaire, Institut Jean-Pierre Bourgin, INRA, 78026 Versailles, France
Max-Planck Institute for Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany
Faculté des Science, Unité Mixte de Recherche 6037, Centre National de la Recherche Scientifique, IFRMP 23, Université de Rouen, 76821 Mont Saint Aignan Cedex, France
Station de Génétique et d'Amélioration des Plantes, Institut Jean-Pierre Bourgin, INRA, 78026 Versailles, France

* Corresponding author; email: Herman.Hofte{at}versailles.inra.fr.

QTL analysis was used to identify genes underlying natural variation in primary cell wall composition in Arabidopsis thaliana. The cell walls of dark-grown seedlings of a Bay-0 x Shahdara recombinant inbred line population were analyzed, using three miniaturized global cell wall fingerprinting techniques: monosaccharide composition analysis by gas-chromatography, xyloglucan oligosaccharide mass profiling and whole-wall Fourier-transform infrared (FTIR) microspectroscopy. Heritable variation and transgression were observed for arabinose/rhamnose ratio, xyloglucan side-chain composition -- including O-acetylation levels -- and absorbance, for a subset of FTIR wavenumbers. In total, 33 QTL, corresponding to at least 11 different loci controlling dark-grown hypocotyl length, pectin composition and levels of xyloglucan fucosylation and O-acetylation, were identified. One major QTL, accounting for 51 % of the variation in arabinose/rhamnose ratio, affected the number of arabinan side chains, presumably attached to the pectic polysaccharide rhamnogalacturonan I, paving the way to positional cloning of the first gene underlying natural variation in pectin structure. Several QTL were found to be colocalized, which may have implications for the regulation of xyloglucan metabolism. These results demonstrate the feasibility of combining fingerprinting techniques, natural variation and quantitative genetics to gain original insights into the molecular mechanisms underlying the structure and metabolism of cell wall polysaccharides.




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