Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on April 21, 2006; 10.1104/pp.106.079418

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
141/2/663    most recent
pp.106.079418v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by M, N.
Right arrow Articles by C, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by M, N.
Right arrow Articles by C, R.
Agricola
Right arrow Articles by M, N.
Right arrow Articles by C, R.

Received February 20, 2006
Returned for revision March 14, 2006
Accepted April 10, 2006

Large scale analysis of mRNA translation states during sucrose starvation in Arabidopsis cells identifies cell proliferation and chromatin structure as targets of translational control

Nicolaï M , Roncato MA , Canoy AS , Rouquié D , Sarda X , Freyssinet G , and Robaglia C *

DSV/DEVM, Laboratoire de Génétique et Biophysique des Plantes, UMR 6191 CEA/CNRS/Université Aix-marseille II, Faculté des Sciences de Luminy, 13009 Marseille Cedex 9, France; Biogemma, 1, rue Pierre Fontaine, 91058 Evry Cedex, France; current address, INRA UGAFL, domaine Saint Maurice, BP 84, 84143 Montfavet Cedex, France
DSV/DEVM, Laboratoire de Génétique et Biophysique des Plantes, UMR 6191 CEA/CNRS/Université Aix-marseille II, Faculté des Sciences de Luminy, 13009 Marseille Cedex 9, France
Biogemma, 1, rue Pierre Fontaine, 91058 Evry Cedex, France
Biogemma, 1, rue Pierre Fontaine, 91058 Evry Cedex, France; current address, Bayer CropScience, 355, rue Dostoïevski, BP 153, 06560 Sophia Antipolis Cedex, France

* Corresponding author; email: robaglia{at}luminy.univ-mrs.fr.

Sucrose starvation of Arabidopsis thaliana cell culture was used to identify translationnally regulated genes by DNA microarray analysis. Cells were starved by subculture without sucrose, and total and polysomal RNA was extracted between 6h and 48h. Probes were derived from both RNA populations and used to screen oligonucleotide microarrays. Out of 25607 screened genes, 224 were found to be differentially accumulated in polysomal RNA following starvation and 21 were found to be invariant in polysomal RNA while their total RNA abundance was modified. Most of the mRNA appears to be translationally repressed (183/245 genes), which is consistent with a general decrease in metabolic activities during starvation. The parallel transcriptional analysis identifies 268 regulated genes. Comparison of transcriptional and translational gene lists highlight the importance of translational regulation (mostly repression) affecting genes involved in cell cycle and cell growth, these being over represented in translationally regulated genes, providing a molecular framework for the arrest of cell proliferation following starvation. Starvation induced translational control also affects chromatin regulation genes, such as the HD1 histone deacetylase, and the level of of histone H4 acetylation was found to increase during starvation. This suggests that regulation of the global nuclear transcriptional activity might be linked to cytoplasmic translational regulations.




This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
M. Hummel, F. Rahmani, S. Smeekens, and J. Hanson
Sucrose-mediated translational control
Ann. Bot., July 1, 2009; 104(1): 1 - 7.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists