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Published on July 7, 2006; 10.1104/pp.106.079509

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Received February 21, 2006
Accepted June 26, 2006

DESENSITIZATION OF GSTF8 INDUCTION BY A PRIOR CHEMICAL TREATMENT IS LONG LASTING AND OPERATES IN A TISSUE-DEPENDENT MANNER

Rhonda C. Foley , Pia G. Sappl , Rafael Perl-Treves , A. Harvey Millar , and Karam B. Singh *

CSIRO Plant Industry, Centre for Environmental and Life Sciences, Private Bag 5, Wembley, WA, 6913. Australia
CSIRO Plant Industry, Centre for Environmental and Life Sciences, Private Bag 5, Wembley, WA, 6913. Australia; ARC Centre of Excellence in Plant Energy Biology and School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Crawley, WA 6009, Australia
ARC Centre of Excellence in Plant Energy Biology and School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Crawley, WA 6009, Australia

* Corresponding author; email: karam.singh{at}csiro.

The Arabidopsis thaliana GSTF8 gene is a member of the glutathione-S-transferase family whose expression is induced by defence signals, certain chemical stresses and some pathogens. Here we have used transgenic plants and an in vivo imaging system to demonstrate GSTF8 expression is subject to a distinct desensitization phenomenon, since prior chemical treatment significantly reduces reactivation of the GSTF8 promoter by hydrogen peroxide, auxin and salicylic acid. A GSTF8 null line had similar desensitization properties as wildtype demonstrating that GSTF8 protein levels are not responsible for desensitization. The resulting refractory period is unusually long lasting, with full recovery taking 4 days. Expression of the GSTF8 promoter following a second treatment occurred predominately in newly formed tissue at the root tip, suggesting that desensitization is lost upon cell division. The expression of the endogenous GSTF8 gene and another GST gene, GSTF6, are also desensitized following treatment with H2O2. The desensitization phenomenon can be activated by a very low concentration of inducer that is not sufficient to activate the GSTF8 promoter. These results demonstrate that activation of the GSTF8 promoter is not essential for eliciting desensitization. A key promoter sequence within the GSTF8 gene, the ocs element, is also affected by desensitization. Treatment with a phosphatase inhibitor prevents desensitization of GSTF8 expression and ocs element activity, suggesting that dephosphorylation of one of more proteins is required for desensitization to occur.




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L. F. Thatcher, C. Carrie, C. R. Andersson, K. Sivasithamparam, J. Whelan, and K. B. Singh
Differential Gene Expression and Subcellular Targeting of Arabidopsis Glutathione S-Transferase F8 Is Achieved through Alternative Transcription Start Sites
J. Biol. Chem., September 28, 2007; 282(39): 28915 - 28928.
[Abstract] [Full Text] [PDF]


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