Plant Physiology Preview
Published on April 21, 2006; 10.1104/pp.106.079574
Received February 23, 2006
Returned for revision March 23, 2006
Accepted April 6, 2006
WRI1 Is Required for Seed Germination and Seedling Establishment
Alex Cernac , Carl Andre , Susanne Hoffmann-Benning , and Christoph Benning *
Dept. of Biochemistry and Molecular Biology, Michigan State University, East Lansing MI 48824, USA
DOE-Plant Research Laboratory, Michigan State University, East Lansing, MI 4 8824, USA; Dept. of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
* Corresponding author; email: benning {at}msu.edu.
Storage compound accumulation during seed development prepares the next generation of plants for survival. Therefore, processes involved in the regulation and synthesis of storage compound accumulation during seed development bear relevance to germination and seedling establishment. The wri1 mutant of Arabidopsis thaliana is impaired in seed oil accumulation. The WRI1 gene encodes an AP2/EREBP transcription factor involved in the control of metabolism, particularly glycolysis, in the developing seeds. Here we investigate the role of this regulatory factor in seed germination and seedling establishment by comparing the wri1-1 mutant, transgenic lines expressing the WRI1 wild-type cDNA in the wri1-1 mutant background, and the wild type. Plants altered in the expression of the WRI1 gene showed different germination responses to the growth factor abscisic acid, sugars and fatty acids provided in the medium. Germination of the mutant was more sensitive to abscisic acid, sugars and osmolites, an effect which was alleviated by increased WRI1 expression in transgenic lines. The expression of abscisic acid responsive genes AtEM6 and ABI3 was increased in the wri1-1 mutant. Double mutant analysis between abi3-3 and wri1-1 suggested that WRI1 and ABI3, a transcription factor mediating abscisic acid responses in seeds, act in parallel pathways. Addition of 2-deoxyglucose inhibited seed germination, but did less so in lines over expressing WRI1. Seedling establishment was decreased in the wri1-1 mutant but could be alleviated by sucrose. Apart from a possible signaling role in germination, sugars in the medium were required as building blocks and energy supply during wri1-1 seedling establishment.
This article has been cited by other articles:
|
|
|
|
 
R. Yano, Y. Kanno, Y. Jikumaru, K. Nakabayashi, Y. Kamiya, and E. Nambara
CHOTTO1, a Putative Double APETALA2 Repeat Transcription Factor, Is Involved in Abscisic Acid-Mediated Repression of Gibberellin Biosynthesis during Seed Germination in Arabidopsis
Plant Physiology,
October 1, 2009;
151(2):
641 - 654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamagishi, K. Tatematsu, R. Yano, J. Preston, S. Kitamura, H. Takahashi, P. McCourt, Y. Kamiya, and E. Nambara
CHOTTO1, a Double AP2 Domain Protein of Arabidopsis thaliana, Regulates Germination and Seedling Growth Under Excess Supply of Glucose and Nitrate
Plant Cell Physiol.,
February 1, 2009;
50(2):
330 - 340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Mu, H. Tan, Q. Zheng, F. Fu, Y. Liang, J. Zhang, X. Yang, T. Wang, K. Chong, X.-J. Wang, et al.
LEAFY COTYLEDON1 Is a Key Regulator of Fatty Acid Biosynthesis in Arabidopsis
Plant Physiology,
October 1, 2008;
148(2):
1042 - 1054.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kondou, M. Nakazawa, M. Kawashima, T. Ichikawa, T. Yoshizumi, K. Suzuki, A. Ishikawa, T. Koshi, R. Matsui, S. Muto, et al.
RETARDED GROWTH OF EMBRYO1, a New Basic Helix-Loop-Helix Protein, Expresses in Endosperm to Control Embryo Growth
Plant Physiology,
August 1, 2008;
147(4):
1924 - 1935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Andre and C. Benning
Arabidopsis Seedlings Deficient in a Plastidic Pyruvate Kinase Are Unable to Utilize Seed Storage Compounds for Germination and Establishment
Plant Physiology,
December 1, 2007;
145(4):
1670 - 1680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zentella, Z.-L. Zhang, M. Park, S. G. Thomas, A. Endo, K. Murase, C. M. Fleet, Y. Jikumaru, E. Nambara, Y. Kamiya, et al.
Global Analysis of DELLA Direct Targets in Early Gibberellin Signaling in Arabidopsis
PLANT CELL,
October 1, 2007;
19(10):
3037 - 3057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Andre, J. E. Froehlich, M. R. Moll, and C. Benning
A Heteromeric Plastidic Pyruvate Kinase Complex Involved in Seed Oil Biosynthesis in Arabidopsis
PLANT CELL,
June 1, 2007;
19(6):
2006 - 2022.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
