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Published on May 12, 2006; 10.1104/pp.106.080309

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Received March 13, 2006
Returned for revision April 12, 2006
Accepted April 26, 2006

Identification of a mung bean arabinofuranosyltransferase that transfers arabinofuranosyl residues onto (1, 5)-linked {alpha}-L-arabino-oligosaccharides

Teruko Konishi , Hiroshi Ono , Mayumi Ohnishi-Kameyama , Satoshi Kaneko , and Tadashi Ishii *

Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan
National Food Research Institute, Tsukuba, Ibaraki, 305-8642, Japan

* Corresponding author; email: tishii{at}ffpri.affrc.go.jp.

Arabinofuranosyltransferase activity was identified in Golgi membranes obtained from mung bean (Vigna radiata) hypocotyls. The enzyme transfers the arabinofuranosyl residue from UDP-{beta}-L-arabinofuranose to exogenous (1, 5)-linked {alpha}-L-arabino-oligosaccharides labeled at their reducing ends with 2-aminobenzamide. The transferred residue was shown, using 1H-NMR spectroscopy and {alpha}-L- arabinofuranosidase treatment, to be {alpha}-L arabinofuranosyl and to be linked to O-5 of the non-reducing terminal arabinofuranosyl residue of the acceptor oligosaccharide. The enzyme was non-processive since only a single arabinofuranosyl residue was added to the acceptor molecule. Arabino-oligosaccharides with a degree of polymerization between 3 and 8 were acceptor substrates. The 2-aminobenzamide labeled arabino-tetra- and pentasaccharides were the most effective acceptor substrates analyzed. The enzyme has a pH optimum between 6.5 and 7.0 and its activity is stimulated by Mn2+ and Co2+ ions. The apparent Km and Vmax values of the arabinofuranosyltransferase for UDP-arabinofuranose are 243 µM, and 243 pmol min-1 mg protein-1, respectively. The highest enzyme activity was detected in the elongating regions of mung bean hypocotyls. The data show that UDP-arabinofuranose is the donor molecule for the generation of arabino-oligosaccharides composed of arabinofuranosyl residues.




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 GlycobiologyHome page
T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi, M. A. O'Neill, and T. Ishii
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
Glycobiology, March 1, 2007; 17(3): 345 - 354.
[Abstract] [Full Text] [PDF]


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