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Published on June 2, 2006; 10.1104/pp.106.080671

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Received March 19, 2006
Returned for revision April 20, 2006
Accepted May 18, 2006

The CLV3/ESR motif of CLV3 is functionally independent from the non-conserved flanking sequences

Martijn Fiers , Elzbieta Golemiec , Roel van der Schors , Lonneke van der Geest , Ka Wan Li , Willem J. Stiekema , and Chun-Ming Liu *

Plant Research International, B.V. P.O. Box 16, 6700 AA Wageningen, The Netherlands
Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Kraków, Poland
Free University, Department of Molecular & Cellular Neurobiology, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
Center for Signal Transduction & Metabolomics, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

* Corresponding author; email: cmliu{at}ibcas.ac.cn.

It is believed that CLAVATA3 (CLV3) encodes a peptide ligand that interacts with the CLV1/CLV2 receptor complex to limit the number of stem cells in the shoot apical meristem (SAM) of Arabidopsis thaliana?however, the exact composition of the functional CLV3 product remains a mystery. A recent study on CLV3 shows that the CLV3/ESR (CLE) motif together with the adjacent C-terminal sequence is sufficient to execute the CLV3 function when fused behind a N-terminal sequence of ERECTA. Here we show that most of the sequences flanking the CLE motif of CLV3 can be deleted without affecting CLV3 function. Using a liquid culture assay, we demonstrate that CLV3p, a synthetic peptide corresponding to the CLE motif of CLV3, is able to restrict the size of the SAM in clv3 seedlings, but not in clv1 seedlings. In accordance with this decrease in meristem size, application of CLV3p to in vitro grown clv3 seedlings restricts the expression of the stem-cell promoting transcription factor WUSCHEL (WUS). Thus, we propose that the CLE motif is the functional region of CLV3 and that this region acts independently of its adjacent sequences.




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