Plant Physiology Preview
Published on June 9, 2006; 10.1104/pp.106.080804
Received March 22, 2006
Returned for revision May 10, 2006
Accepted May 30, 2006
Pathways of glucose regulation of monosaccharide transport in grape cells
Carlos Conde , Alice Agasse , David Glissant , Rui Tavares , Hernâni Gerós *, and Serge Delrot
Departamento de Biologia, Universidade do Minho, Campus de Gualtar 4710-057 Braga, Portugal
UMR CNRS 6161, Transport des Assimilats, Laboratoire de Physiologie, Biochimie et Biologie Moléculaires Végétales, Bâtiment Botanique, UFR Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers Cédex, France
* Corresponding author; email: geros{at}bio.uminho.pt.
Vitis vinifera heterotrophic suspension cultured cells were used as a model system to study glucose (Glc) transport and its regulation. Cells transported D-[14C]glucose according to simple Michaelis-Menten kinetics superimposed to a first-order kinetics. The saturating component is a high affinity, broad specificity H+-dependent transport system (K
m, 0.05 mM). Glc concentration in the medium tightly regulated the transcription of VvHT1, a monosaccharide transporter previously characterized in grape berry, as well as VvHT1 protein amount and monosaccharide transport activity. All the remaining putative monosaccharide transporters identified so far in grape were poorly expressed and responded weakly to Glc. VvHT1 transcription was strongly repressed by Glc, and 2-deoxy-D-glucose, but neither by 3-O-methyl-D-glucose (3-O-MG) nor by Glc plus mannoheptulose (MHL), indicating the involvement of a hexokinase-dependent repression. 3-O-MG, which cannot be phosphorylated, and Glc plus MHL induced a decrease of transport activity caused by the reduction of VvHT1 protein in the plasma membrane, without affecting VvHT1 transcript levels. This demonstrates a hexokinase-independent posttranscriptional regulation. High Glc down-regulated VvHT1 transcription and Glc uptake, whereas low Glc increased those parameters. Present data provide the first example showing control of plant sugar transporters by its own substrate both at transcriptional and posttranscriptional levels. VvHT1 protein has an important role in the massive import of monosaccharides into mesocarp cells of young grape berries since it was localized in plasma membranes of the early developing fruit. Protein amount decreased abruptly throughout fruit development as sugar content increases, consistent with the regulating role of Glc on VvHT1 expression found in suspension cultured cells.
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