Plant Physiology Preview
Published on June 23, 2006; 10.1104/pp.106.081018
Received March 29, 2006
Returned for revision May 7, 2006
Accepted June 8, 2006
Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1
Angela Saez , Nadia Robert , Mohammad H. Maktabi , Julian I. Schroeder , Ramón Serrano , and Pedro L. Rodriguez *
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, E-46022 Valencia, Spain
Cell and Developmental Biology Section, Division of Biological Sciences, and Center for Molecular genetics, University of California, San Diego, La Jolla, California 92093-0116, USA
* Corresponding author; email: prodriguez{at}ibmcp.upv.es.
Abscisic acid plays a key role in plant responses to abiotic stress, particularly drought stress. A wide number of ABA-hypersensitive mutants is known, however only a few of them resist/avoid drought stress. In this work we have generated ABA-hypersensitive drought-avoidant mutants by simultaneous inactivation of two negative regulators of ABA signaling, i.e. the protein phosphatases type 2C (PP2Cs) ABI1 and HAB1. Two new recessive loss-of-function alleles of ABI1, abi1-2 and abi1-3, were identified in an Arabidopsis thaliana T-DNA collection. These mutants showed enhanced responses to ABA both in seed and vegetative tissues, but only a limited effect on plant drought avoidance. In contrast, generation of double hab1-1abi1-2 and hab1-1abi1-3 mutants strongly increased plant responsiveness to ABA. Thus, both hab1-1abi1-2 and hab1-1abi1-3 were particularly sensitive to ABA-mediated inhibition of seed germination. Additionally, vegetative responses to ABA were reinforced in the double mutants, which showed a strong hypersensitivity to ABA in growth assays, stomatal closure and induction of ABA-responsive genes. Transpirational water loss under drought conditions was noticeably reduced in the double mutants as compared to single parental mutants, which resulted in reduced water consumption of whole plants. Taken together, these results reveal cooperative negative regulation of ABA signaling by ABI1 and HAB1 and suggest that fine tuning of ABA signaling can be attained through combined action of PP2Cs. Finally, these results suggest that combined inactivation of specific PP2Cs involved in ABA signaling could provide an approach for improving crop performance under drought stress conditions.
This article has been cited by other articles:
|
|
|
|
 
J. Guo, J. Wang, L. Xi, W.-D. Huang, J. Liang, and J.-G. Chen
RACK1 is a negative regulator of ABA responses in Arabidopsis
J. Exp. Bot.,
September 1, 2009;
60(13):
3819 - 3833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wan, R. Griffiths, J. Ying, P. McCourt, and Y. Huang
Development of Drought-Tolerant Canola (Brassica napus L.) through Genetic Modulation of ABA-mediated Stomatal Responses
Crop Sci.,
August 7, 2009;
49(5):
1539 - 1554.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rubio, A. Rodrigues, A. Saez, M. B. Dizon, A. Galle, T.-H. Kim, J. Santiago, J. Flexas, J. I. Schroeder, and P. L. Rodriguez
Triple Loss of Function of Protein Phosphatases Type 2C Leads to Partial Constitutive Response to Endogenous Abscisic Acid
Plant Physiology,
July 1, 2009;
150(3):
1345 - 1355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ma, I. Szostkiewicz, A. Korte, D. Moes, Y. Yang, A. Christmann, and E. Grill
Regulators of PP2C Phosphatase Activity Function as Abscisic Acid Sensors
Science,
May 22, 2009;
324(5930):
1064 - 1068.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Park, P. Fung, N. Nishimura, D. R. Jensen, H. Fujii, Y. Zhao, S. Lumba, J. Santiago, A. Rodrigues, T.-f. F. Chow, et al.
Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins
Science,
May 22, 2009;
324(5930):
1068 - 1071.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rodrigues, J. Santiago, S. Rubio, A. Saez, K. S. Osmont, J. Gadea, C. S. Hardtke, and P. L. Rodriguez
The Short-Rooted Phenotype of the brevis radix Mutant Partly Reflects Root Abscisic Acid Hypersensitivity
Plant Physiology,
April 1, 2009;
149(4):
1917 - 1928.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Wang, M. Beaith, M. Chalifoux, J. Ying, T. Uchacz, C. Sarvas, R. Griffiths, M. Kuzma, J. Wan, and Y. Huang
Shoot-Specific Down-Regulation of Protein Farnesyltransferase ({alpha}-Subunit) for Yield Protection against Drought in Canola
Mol Plant,
January 1, 2009;
2(1):
191 - 200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhang, W. Xu, Z. Li, X. W. Deng, W. Wu, and Y. Xue
F-Box Protein DOR Functions As a Novel Inhibitory Factor for Abscisic Acid-Induced Stomatal Closure under Drought Stress in Arabidopsis
Plant Physiology,
December 1, 2008;
148(4):
2121 - 2133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Saez, A. Rodrigues, J. Santiago, S. Rubio, and P. L. Rodriguez
HAB1-SWI3B Interaction Reveals a Link between Abscisic Acid Signaling and Putative SWI/SNF Chromatin-Remodeling Complexes in Arabidopsis
PLANT CELL,
November 1, 2008;
20(11):
2972 - 2988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Wasilewska, F. Vlad, C. Sirichandra, Y. Redko, F. Jammes, C. Valon, N. F. d. Frey, and J. Leung
An Update on Abscisic Acid Signaling in Plants and More ...
Mol Plant,
March 1, 2008;
1(2):
198 - 217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Jung, J. S. Seo, S. W. Han, Y. J. Koo, C. H. Kim, S. I. Song, B. H. Nahm, Y. D. Choi, and J.-J. Cheong
Overexpression of AtMYB44 Enhances Stomatal Closure to Confer Abiotic Stress Tolerance in Transgenic Arabidopsis
Plant Physiology,
February 1, 2008;
146(2):
623 - 635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Nilson and S. M. Assmann
The Control of Transpiration. Insights from Arabidopsis
Plant Physiology,
January 1, 2007;
143(1):
19 - 27.
[Full Text]
[PDF]
|
|
|
|
|
