Plant Physiology Preview
Published on April 28, 2006; 10.1104/pp.106.081208
OPEN ACCESS ARTICLE
Received March 30, 2006
Returned for revision April 3, 2006
Accepted April 6, 2006
Combined transcript and metabolite profiling of Arabidopsis leaves reveals fundamental effects of the thiol-disulfide status on plant metabolism
Anna Kolbe , Sandra Oliver , Alisdair R. Fernie , Mark Stitt , Joost T. van Dongen , and Peter Geigenberger *
Max-Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm-Potsdam, Germany
* Corresponding author; email: geigenberger{at}mpimp-golm.mpg.de.
In the present paper, we used GC-MS analysis in combination with flux analysis and the Affymetrix ATH1 GeneChip to survey the metabolome and transcriptome of Arabidopsis leaves in response to manipulation of the thiol-disulfide status. Feeding low concentrations of the sulfhydryl reagent dithiothreitol (DTT) for one hour at the end of the dark period led to post-translational redox-activation of ADP-glucose pyrophosphorylase and major alterations in leaf carbon partitioning, including an increased flux into major respiratory pathways, starch- cell-wall-, and amino-acid synthesis and a reduced flux to sucrose. This was accompanied by a decrease in the levels of hexose-phosphates, while metabolites in the second half of the TCA cycle and various amino acids increased, indicating a stimulation of anaplerotic fluxes reliant on  -ketoglutarate. There was also an increase in shikimate as a precursor of secondary plant products and marked changes in the levels of the minor sugars involved in ascorbate synthesis and cell wall metabolism. Transcript profiling revealed a relatively small number of changes in the levels of transcripts coding for components of redox-regulation, transport processes and cell wall, protein and amino acid metabolism, while there were no major alterations in transcript levels coding for enzymes involved in central metabolic pathways. These results provide a global picture of the effect of redox and reveal the utility of transcript and metabolite profiling as systemic strategies to uncover the occurrence of redox-modulation in vivo.
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