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Published on July 14, 2006; 10.1104/pp.106.081752

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Received April 7, 2006
Accepted July 11, 2006

Expression of CAP2, an AP2-family Transcription Factor from Chickpea Enhances Growth and Tolerance to Dehydration and Salt Stress in Transgenic Tobacco

Rakesh K. Shukla , Sumita Raha , Vineeta Tripathi , and Debasis Chattopadhyay *

National Centre for Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India

* Corresponding author; email: debasis_chattopadhyay{at}yahoo.co.in.

The AP2 domain defines a large family of DNA-binding proteins that play important roles in plant morphology, development and stress response. We describe isolation and characterization of a gene (CAP2) from Cicer arietinum (Chickpea) encoding a novel AP2-family transcription factor. Recombinant CAP2 protein bound specifically to C-repeat/Dehydration responsive element in gel shift assay and transactivated reporter genes in yeast one-hybrid assay. CAP2 appeared to be a single/low copy intron-less gene and the protein product localized in the nucleus. Transcript level of CAP2 increased by dehydration and by treatment with sodium chloride, abscisic acid and auxin, but not by treatment with low temperature, salicylic acid and jasmonic acid. 35S promoter-driven expression of CAP2 in tobacco caused drastic increase in the leaf cell size, and thereby, in leaf surface area and in the number of lateral roots. Transgenic plants demonstrated more tolerance to dehydration and salt stress than the wild type plants. Transgenic plants expressed higher steady state transcript levels of abiotic stress response genes NtERD10B and NtERD10C; and auxin-response genes IAA4.2 and IAA2.5. Taken together, our results indicated a mutual interrelation between plant growth-development and abiotic stress response pathways and a probable involvement of CAP2 in both the signaling pathways.




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