Plant Physiology Preview
Published on August 4, 2006; 10.1104/pp.106.082628
Received April 27, 2006
Accepted July 26, 2006
Receptor Signal Output Mediated by the ETR1 N-Terminus Is Primarily Subfamily I Receptors-Dependent
Fang Xie , Qian Liu , and Chi-Kuang Wen *
National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China
* Corresponding author; email: cwen{at}sippe.ac.cn; qgwen@sibs.ac.cn.
etr1-1is a dominant ethylene receptor gene in Arabidopsis and confers ethylene insensitivity. The truncated etr1-1(1-349) protein is capable of repressing ethylene responses while etr1(1-349) is not, lending support to a hypothesis that the dominant etr1-1(1-349) could convert wild-type receptors to an ethylene-insensitive state. Assuming that etr1-1(1-349) and etr1(1-349) would share a same signaling mechanism, we hypothesize that the etr1(1-349) protein is capable of repressing ethylene responses when not bound with ethylene. In this study, we show that both etr1(1-349) and etr1-1(1-349) are capable of receptor signal output which is primarily dependent of subfamily I receptors. The etr1(1-349) and etr1-1(1-349) clones were individually transformed to mutants and the resulting phenotypes were scored. Each of those transgenes restored the rosette growth and flower fertility of etr1-7 ers1-2 to a similar extent. In contrast, neither etr1(1-349) nor etr1-1(1-349) was capable of signal output in etr1-7 ers1-3. The ERS1 transcript was detectable in ers1-2 but not in ers1-3, implying that the ETR1 N-terminus signaling is subfamily I-dependent. Loss of the subfamily II receptor genes did not perturb the etr1-1(1-349)-mediated ethylene insensitivity. Possible roles of subfamily I receptors and disulfide linkages in the ETR1 receptor signal output mediated through the N-terminus are discussed.
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