Plant Physiology Preview
Published on June 15, 2006; 10.1104/pp.106.082941
Received May 2, 2006
Returned for revision June 6, 2006
Accepted June 7, 2006
A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice (Oryza sativa L.)
Zhaosheng Kong , Meina Li , Wenqiang Yang , Wenying Xu , and Yongbiao Xue *
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100080, China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, China; College of Life Sciences, Tianjin Normal University, Tianjin, 300074, China
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100080, China; Graduate University of Chinese Academy of Sciences, Beijing, 100049, China
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100080, China
* Corresponding author; email: ybxue{at}genetics.ac.cn.
Leaf senescence is a developmentally programmed degeneration process, which is fine-tuned by a complex regulatory network for plant fitness. However, molecular regulation of leaf senescence is poorly understood, especially in rice (Oryza sativa L.), an important staple crop for more than half of the world population. Here, we report a novel nuclear-localized CCCH-type zinc finger protein, OsDOS, involved in delaying leaf senescence in rice. The expression of OsDOS was down-regulated during natural leaf senescence, panicle development and pollination, although its transcripts were accumulated in various organs. RNAi knockdown of OsDOS caused an accelerated age-dependent leaf senescence, whereas its overexpression produced a marked delay of leaf senescence, suggesting that it acts as a negative regulator for leaf senescence. A genome-wide expression analysis further confirmed its negative regulation for leaf senescence and revealed that, in particular, the jasmonate (JA) pathway was found to be hyperactive in the OsDOS RNAi transgenic lines but impaired in the OsDOS overexpressing transgenic lines, indicating that this pathway is likely involved in the OsDOS-mediated delaying of leaf senescence. Furthermore, methyl jasmonate (MeJA) treatments of both seeds and detached leaves from the RNAi and the overexpressing transgenic lines showed hyper- and hypo-responses, respectively, consistent with the negative regulation of the JA pathway by OsDOS. Together, these results indicate that OsDOS is a novel nuclear protein that delays leaf senescence likely, at least in part, by integrating developmental cues to the JA pathway.
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