Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on July 28, 2006; 10.1104/pp.106.082982

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
142/1/333    most recent
pp.106.082982v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eisenhut, M.
Right arrow Articles by Hagemann, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eisenhut, M.
Right arrow Articles by Hagemann, M.
Agricola
Right arrow Articles by Eisenhut, M.
Right arrow Articles by Hagemann, M.

Received May 2, 2006
Accepted July 24, 2006

The Plant-like C2 Glycolate Pathway and the Bacterial-like Glycerate Cycle Cooperate in Phosphoglycolate Metabolism in Cyanobacteria

Marion Eisenhut , Shira Kahlon , Dirk Hasse , Ralph Ewald , Judy Lieman-Hurwitz , Teruo Ogawa , Wolfgang Ruth , Hermann Bauwe , Aaron Kaplan , and Martin Hagemann *

Universität Rostock, Institut Biowissenschaften, Pflanzenphysiologie, Einsteinstr. 3, D-18051 Rostock, Germany
Department of Plant and Environmental Sciences, The Hebrew University of Jerusalem, Israel
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200032, China
Universität Rostock, Institut für Chemie, Einsteinstr. 3a, D-18059 Rostock, Germany

* Corresponding author; email: martin.hagemann{at}uni-rostock.de.

The occurrence of a photorespiratory 2-phosphoglycolate metabolism in cyanobacteria is not clear. In the genome of the cyanobacterium Synechocystis sp. strain PCC 6803 we have identified open reading frames encoding enzymes homologous to those forming the plant-like C2 cycle and the bacterial-type glycerate pathway. To study the route and importance of 2-phosphoglycolate metabolism, the identified genes were systematically inactivated by mutagenesis. With a few exceptions, most of these genes could be inactivated without leading to a high CO2-requiring phenotype. Biochemical characterization of recombinant proteins verified that Synechocystis harbors an active serine hydroxymethyltransferase and, contrary to higher plants, expresses a glycolate dehydrogenase instead of an oxidase to convert glycolate to glyoxylate. The mutation of this enzymatic step, located prior to the branching of phosphoglycolate metabolism into the plant-like C2 cycle and the bacterial-like glycerate pathway, resulted in glycolate accumulation and a growth depression already at high CO2. Similar growth inhibitions were found for a single mutant in the plant-type C2 cycle and more pronounced for a double mutant affected in both the C2 cycle and the glycerate pathway after cultivation at low CO2. These results suggested that cyanobacteria metabolize phosphoglycolate by the cooperative action of the C2 cycle and the glycerate pathway. When exposed to low CO2, glycine decarboxylase knockout mutants accumulated far more glycine and lysine than wild type cells or mutants with inactivated glycerate pathway. This finding and the growth data imply a dominant although not exclusive role of the C2 route in cyanobacterial phosphoglycolate metabolism.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Eisenhut, W. Ruth, M. Haimovich, H. Bauwe, A. Kaplan, and M. Hagemann
The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants
PNAS, November 4, 2008; 105(44): 17199 - 17204.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Xu, G. Bernat, A. Singh, H. Mi, M. Rogner, H. B. Pakrasi, and T. Ogawa
Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems
Plant Cell Physiol., November 1, 2008; 49(11): 1672 - 1677.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. D. Price, M. R. Badger, F. J. Woodger, and B. M. Long
Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants
J. Exp. Bot., May 1, 2008; 59(7): 1441 - 1461.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Ewald, U. Kolukisaoglu, U. Bauwe, S. Mikkat, and H. Bauwe
Mitochondrial Protein Lipoylation Does Not Exclusively Depend on the mtKAS Pathway of de Novo Fatty Acid Synthesis in Arabidopsis
Plant Physiology, September 1, 2007; 145(1): 41 - 48.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Eisenhut, E. A. von Wobeser, L. Jonas, H. Schubert, B. W. Ibelings, H. Bauwe, H. C.P. Matthijs, and M. Hagemann
Long-Term Response toward Inorganic Carbon Limitation in Wild Type and Glycolate Turnover Mutants of the Cyanobacterium Synechocystis sp. Strain PCC 6803
Plant Physiology, August 1, 2007; 144(4): 1946 - 1959.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists