Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on August 11, 2006; 10.1104/pp.106.083519

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
142/2/651    most recent
pp.106.083519v2
pp.106.083519v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jin, H.
Right arrow Articles by Villegas, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jin, H.
Right arrow Articles by Villegas, A., Jr.
Agricola
Right arrow Articles by Jin, H.
Right arrow Articles by Villegas, A.

Received May 12, 2006
Accepted August 2, 2006

Down Regulation of 26S Proteasome Subunit RPN9 Inhibits Viral Systemic Transport and Alters Plant Vascular Development

Hailing Jin *, Songtao Li , and Andy Villegas Jr.

Center for Plant Cell Biology, Department of Plant Pathology, University of California, Riverside, CA 92521, USA

* Corresponding author; email: hailingj{at}ucr.edu.

Plant viruses utilize the vascular system for systemic movement. The plant vascular network also transports water, photosynthates, and signaling molecules and is essential for plant growth. However, the molecular mechanisms governing vascular development and patterning are still largely unknown. From a viral transport suppressor screening using virus-induced gene silencing (VIGS), we identified a 26S proteasome subunit, RPN9, which is required for broad-spectrum viral systemic transport. Silencing of RPN9 in N. benthamiana inhibits systemic spread of two taxonomically distinct viruses, tobacco mosaic virus (TMV) and turnip mosaic virus (TuMV). The 26S proteasome is a highly conserved eukaryotic protease complex controlling many fundamental biochemical processes, but the functions of many 26S proteasome regulatory subunits, especially in plants, are still poorly understood. We demonstrate that the inhibition of viral systemic transport after RPN9 silencing is largely due to alterations in the vascular tissue. RPN9-silenced plants display extra leaf vein formation with increased xylem and decreased phloem. We further illustrate that RPN9 functions at least in part through regulation of auxin transport and Brassinosteroid (BR) signaling, two processes that are crucial for vascular formation. We propose that RPN9 regulates vascular formation by targeting a subset of regulatory proteins for degradation. The BR signaling protein BZR1 is one of the targets.




This article has been cited by other articles:


Home page
 DevelopmentHome page
K. Marrocco, A. Thomann, Y. Parmentier, P. Genschik, and M. C. Criqui
The APC/C E3 ligase remains active in most post-mitotic Arabidopsis cells and is required for proper vasculature development and organization
Development, May 1, 2009; 136(9): 1475 - 1485.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists