Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on June 9, 2006; 10.1104/pp.106.084079

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
141/4/1544    most recent
pp.106.084079v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ellis, D. R
Right arrow Articles by Salt, D. E
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ellis, D. R
Right arrow Articles by Salt, D. E
Agricola
Right arrow Articles by Ellis, D. R
Right arrow Articles by Salt, D. E

Received May 24, 2006
Returned for revision June 1, 2006
Accepted June 1, 2006

A NOVEL ARSENATE REDUCTASE FROM THE ARSENIC HYPERACCUMULATING FERN PTERIS VITTATA

Danielle R Ellis , Luke Gumaelius , Emily Indriolo , Ingrid J Pickering , Jo Ann Banks *, and David E Salt

Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907
Department of Geological Sciences, University of Saskatchewan, Saskatoon, SK S7N 5E2
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907

* Corresponding author; email: banksj{at}purdue.edu.

Pteris vittata L. sporophytes hyperaccumulate arsenic to 1-2% of their dry weight. Like the sporophyte, the gametophyte was found to reduce arsenate (As(V)) to arsenite (As(III)) and store arsenic as free As(III). Here we report the isolation of an arsenate reductase gene (PvACR2) from gametophytes that can suppress the arsenate sensitivity and arsenic hyper-accumulation phenotypes of Saccharomyces cerevisiae lacking the arsenate reductase gene ScACR2. Recombinant PvACR2 protein has in vitro arsenate reductase activity similar to ScACR2. While PvACR2 and ScACR2 have sequence similarities to the CDC25 protein tyrosine phosphatases, they lack phosphatase activity. In contrast, Arath;CDC25, an Arabidopsis thaliana homolog of PvACR2 was found to have both arsenate reductase and phosphatase activities. PvACR2 is the first reported plant arsenate reductase that lacks phosphatase activity. CDC25 protein tyrosine phosphatases and arsenate reductases have a conserved HCX5R motif that defines the active site. PvACR2 is unique in that the arginine of this motif, previously shown to be essential for phosphatase and reductase activity, is replaced with a serine. Steady-state levels of PvACR2 expression in gametophytes were found to be similar in the absence and presence of arsenate while total arsenate reductase activity in P. vittata gametophytes was found to be constitutive and unaffected by arsenate, consistent with other known metal hyperaccumulation mechanisms in plants. The unusual active site of PvACR2 and the arsenate reductase activities of cell free extracts correlate with the ability of P. vittata to hyperaccumulate arsenite, suggesting that PvACR2 may play an important role in this process.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Sundaram, B. Rathinasabapathi, L. Q. Ma, and B. P. Rosen
An Arsenate-activated Glutaredoxin from the Arsenic Hyperaccumulator Fern Pteris vittata L. Regulates Intracellular Arsenite
J. Biol. Chem., March 7, 2008; 283(10): 6095 - 6101.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Kerk, G. Templeton, and G. B.G. Moorhead
Evolutionary Radiation Pattern of Novel Protein Phosphatases Revealed by Analysis of Protein Data from the Completely Sequenced Genomes of Humans, Green Algae, and Higher Plants
Plant Physiology, February 1, 2008; 146(2): 351 - 367.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists