Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on June 30, 2006; 10.1104/pp.106.084178

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
141/4/1425    most recent
pp.106.084178v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mo, X.
Right arrow Articles by Wu, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mo, X.
Right arrow Articles by Wu, P.
Agricola
Right arrow Articles by Mo, X.
Right arrow Articles by Wu, P.

Received May 25, 2006
Accepted June 16, 2006

The hpa1 mutant of Arabidopsis reveals a crucial role of histidine homeostasis in root meristem maintenance

Xiaorong Mo , Qingyu Zhu , Xiang Li , Jing Li , Qingning Zeng , Honglin Rong , Hanma Zhang *, and Ping Wu

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, 310029, P. R. China
Institute of Integrative & Comparative Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK

* Corresponding author; email: bgyhz{at}leeds.ac.uk.

Histidine is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that histidine is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here we present evidence for such a role of this amino acid in root development in Arabidopsis from the characterization of a novel Arabidopsis mutant, hpa1, which has a very short root system and carries a mutation in one of the two Arabidopsis histidinol phosphate aminotransferase (HPA) genes, AtHPA1. We have established that AtHPA1 encodes a functional HPA and that its complete knockout is embryo lethal. Biochemical analysis shows that the mutation in hpa1only resulted in a 30% reduction in free histidine content and had no significant impact on the total histidine content. It did not cause any known symptoms of histidine starvation. However, the mutant displayed a specific developmental defect in root meristem maintenance and was unable to sustain primary root growth 2 days after germination. We have demonstrated that the root meristem failure in the mutant is tightly linked to the reduction in free histidine content and could be rescued by either exogenous histidine supplementation or AtHPA1 over-expression. Our results therefore reveal an important role of histidine homeostasis in plant development.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. Munoz-Bertomeu, B. Cascales-Minana, J. M. Mulet, E. Baroja-Fernandez, J. Pozueta-Romero, J. M. Kuhn, J. Segura, and R. Ros
Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Deficiency Leads to Altered Root Development and Affects the Sugar and Amino Acid Balance in Arabidopsis
Plant Physiology, October 1, 2009; 151(2): 541 - 558.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. Bikard, D. Patel, C. Le Mette, V. Giorgi, C. Camilleri, M. J. Bennett, and O. Loudet
Divergent Evolution of Duplicate Genes Leads to Genetic Incompatibilities Within A. thaliana
Science, January 30, 2009; 323(5914): 623 - 626.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. G. Forde and P. J. Lea
Glutamate in plants: metabolism, regulation, and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2339 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Muralla, C. Sweeney, A. Stepansky, T. Leustek, and D. Meinke
Genetic Dissection of Histidine Biosynthesis in Arabidopsis
Plant Physiology, June 1, 2007; 144(2): 890 - 903.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists