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Published on July 14, 2006; 10.1104/pp.106.084319

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Received May 30, 2006
Accepted July 10, 2006

The TATA-box Sequence in Basal Promoter Contributes to Determining the Light Dependent Gene Expression in Plants

Kanti Kiran , Suraiya A. Ansari , Rakesh Srivastava , Niraj Lodhi , Chandra Prakash Chaturvedi , Samir V. Sawant , and Rakesh Tuli *

National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226001, India

* Corresponding author; email: rakeshtuli{at}hotmail.com.

A prototype 13 bp TATA-box sequence TCACTATATATAG was mutated at each nucleotide position and examined for its function in a core promoter. Specific nucleotides in the first TATA, the second TATA as well as the flanking sequences influenced promoter function in transient transformation of Nicotiana tabaccum var Petit Havana leaves. The effect of a given mutation on reporter gene expression in light versus dark was variable and sometimes contrasting. Some mutations, like T7 or A8 -> C or G completely inactivated the expression of the minimal promoter in light but not in dark. In general, the sequence requirement for dark - expression was less stringent than that for the expression in light. The selective effect of the TATA-box mutations on light versus dark expression was exerted on core promoter function in chromatin integrated state also. Even in the presence of an upstream light response activator element, the TATA-box mutations influenced modulation of the promoter by light. An A at the 8th position was specifically involved in red light response of the promoter. The selectivity in gene expression was associated with a high level of transcript initiation from a site that was not active in the dark. Nuclear proteins from dark and light grown seedlings showed that the sequence variation within TATA-box governs the formation of alternative transcriptional complexes. The experiments give direct evidence for the role of core TATA-box sequence in determining the level as well as selectivity of gene expression in plants.




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Y. Y. Yamamoto, H. Ichida, T. Abe, Y. Suzuki, S. Sugano, and J. Obokata
Differentiation of core promoter architecture between plants and mammals revealed by LDSS analysis
Nucleic Acids Res., September 25, 2007; 35(18): 6219 - 6226.
[Abstract] [Full Text] [PDF]


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