Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on July 28, 2006; 10.1104/pp.106.084533

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
142/1/63    most recent
pp.106.084533v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ito, H.
Right arrow Articles by Gray, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ito, H.
Right arrow Articles by Gray, W. M.
Agricola
Right arrow Articles by Ito, H.
Right arrow Articles by Gray, W. M.

Received June 2, 2006
Accepted July 26, 2006

A Gain-of-function Mutation in the Arabidopsis Pleiotropic Drug Resistance Transporter PDR9 Confers Resistance to Auxinic Herbicides

Hironori Ito and William M. Gray *

Department of Plant Biology, University of Minnesota-Twin Cities, St. Paul, MN 55108

* Corresponding author; email: grayx051{at}tc.umn.edu.

Arabidopsis contains 15 genes encoding members of the pleiotropic drug resistance (PDR) family of ABC transporters. These proteins have been speculated to be involved in the detoxification of xenobiotics, however little experimental support of this hypothesis has been obtained to date. Here we report our characterization of the Arabidopsis PDR9 gene. We isolated a semi-dominant, gain-of-function mutant, designated pdr9-1, that exhibits increased tolerance to the auxinic herbicide 2,4-D. Reciprocally, loss-of-function mutations in PDR9 confer 2,4-D hypersensitivity. This altered auxin sensitivity defect of pdr9 mutants is specific for 2,4-D and closely related compounds as these mutants respond normally to the endogenous auxins IAA and IBA. We demonstrate that 2,4-D, but not IAA, transport is affected by mutations in pdr9 suggesting that the PDR9 transporter specifically effluxes 2,4-D out of plant cells without affecting endogenous auxin transport. The semi-dominant pdr9-1 mutation affects an extremely highly conserved domain present in all known plant PDR transporters. The single amino acid change results in increased PDR9 abundance and provides a novel approach for elucidating the function of plant PDR proteins.




This article has been cited by other articles:


Home page
 Plant CellHome page
L. C. Strader and B. Bartel
The Arabidopsis PLEIOTROPIC DRUG RESISTANCE8/ABCG36 ATP Binding Cassette Transporter Modulates Sensitivity to the Auxin Precursor Indole-3-Butyric Acid
PLANT CELL, July 1, 2009; 21(7): 1992 - 2007.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. A. Ramirez-Carvajal, A. M. Morse, C. Dervinis, and J. M. Davis
The Cytokinin Type-B Response Regulator PtRR13 Is a Negative Regulator of Adventitious Root Development in Populus
Plant Physiology, June 1, 2009; 150(2): 759 - 771.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. C. Strader, M. Monroe-Augustus, K. C. Rogers, G. L. Lin, and B. Bartel
Arabidopsis iba response5 Suppressors Separate Responses to Various Hormones
Genetics, December 1, 2008; 180(4): 2019 - 2031.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Cho, S. H. Lee, and H.-T. Cho
P-Glycoprotein4 Displays Auxin Efflux Transporter-Like Action in Arabidopsis Root Hair Cells and Tobacco Cells
PLANT CELL, December 1, 2007; 19(12): 3930 - 3943.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. W. Woodward, S. E. Ratzel, E. E. Woodward, Y. Shamoo, and B. Bartel
Mutation of E1-CONJUGATING ENZYME-RELATED1 Decreases RELATED TO UBIQUITIN Conjugation and Alters Auxin Response and Development
Plant Physiology, June 1, 2007; 144(2): 976 - 987.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists