Plant Physiology Preview
Published on August 11, 2006; 10.1104/pp.106.084871
Received June 8, 2006
Accepted August 3, 2006
Transcriptional Regulation of Gibberellin Metabolism Genes by Auxin Signaling in Arabidopsis
Martín Frigerio , David Alabadí , José Pérez-Gómez , Laura García-Cárcel , Andrew L. Phillips , Peter Hedden , and Miguel A. Blázquez *
Instituto de Biología Molecular y Celular de Plantas (UPV-CSIC), Universidad Politécnica de Valencia, 46022 Valencia, Spain
Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK
* Corresponding author; email: mblazquez{at}ibmcp.upv.es.
Auxin and GAs overlap in the regulation of multiple aspects of plant development, such as root growth and organ expansion. This coincidence raises the question of whether these two hormones interact to regulate common targets, and what type of interaction occurs in each case. Auxins induce GA biosynthesis in a range of plant species. We have undertaken a detailed analysis of the auxin regulation of expression of Arabidopsis genes encoding GA 20-oxidases and 3-oxidases, involved in GA biosynthesis, and GA 2-oxidases, involved in GA inactivation. Our results show that auxin differentially upregulates the expression of various genes involved in GA metabolism, in particular several AtGA20ox and AtGA2ox genes. Upregulation occurred very quickly after auxin application; the response was mimicked by incubations with the protein synthesis inhibitor cycloheximide, and was blocked by treatments with the proteasome inhibitor MG132. The effects of auxin treatment reflect endogenous regulation since equivalent changes in gene expression were observed in the auxin over-producer mutant yucca. The results suggest direct regulation of the expression of GA metabolism genes by Aux/IAA and ARF proteins. The physiological relevance of this regulation is supported by the observation that the phenotype of certain gain-of-function Aux/IAA alleles could be alleviated by GA application, which suggests that changes in GA metabolism mediate part of auxin action during development.
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