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Published on September 8, 2006; 10.1104/pp.106.085043

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Received June 12, 2006
Accepted August 30, 2006

Building-up of the Plastid Transcriptional Machinery during Germination and Early Plant Development

Emilie Demarsy , Florence Courtois , Jacinthe Azevedo , Laurence Buhot , and Silva Lerbs-Mache *

Laboratoire Plastes et Differenciation cellulaire, Université Joseph Fourier and Centre National de la Recherche Scientifique, B.P. 53, F-38041 Grenoble, France

* Corresponding author; email: silva.lerbs-mache{at}ujf-grenoble.fr.

The plastid genome is transcribed by three different RNA polymerases, one is called PEP (plastid-encoded RNA polymerase) and two are called NEPs (nucleus-encoded RNA polymerases). PEP transcribes preferentially photosynthesis-related genes in mature chloroplasts while NEP transcribes preferentially housekeeping genes during early phases of plant development, and it was generally thought that during plastid differentiation the building-up of the NEP transcription system precedes the building-up of the PEP transcription system. We have now analysed in detail the establishment of the two different transcription systems, NEP and PEP, during germination and early seedling development on the mRNA and protein level. Experiments have been performed with two different plant species, Arabidopsis thaliana and Spinacia oleracea. Results show that the building-up of the two different transcription systems is different in the two species. However, in both species NEP as well as PEP is already present in seeds, and results using Tagetin as specific inhibitor of PEP activity demonstrate that PEP is important for efficient germination, i. e. PEP is already active in not yet photosynthetically active seed plastids.




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