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Published on August 18, 2006; 10.1104/pp.106.085068

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Received June 12, 2006
Accepted July 21, 2006

Detection and Quantification of Unbound Phytochelatin 2 (PC2) in Plant Extracts of Brassica napus Grown with Different Levels of Mercury

Iglesia-Turiño Santiago , Anna Febrero *, Olga Jauregui , Cristina Caldelas , Jose Luis Araus , and Jordi Bort

Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 645, 08028 Barcelona, Spain.
Scientific and Technical Services, University of Barcelona, 08028 Barcelona, Spain

* Corresponding author; email: annafebrero{at}ub.edu.

The mercury accumulation mechanism was studied in Brassica napus (rape) plants grown under a mercury concentration gradient (from 0µM to 1000µM). Mercury mainly accumulated in roots. Therefore, the presence of Phytochelatins (PC) was studied in the roots of the plants. The high stability of the phytochelatin-mercury multicomplexes (mPC-nHg) seems to be the main reason for the lack of previous Hg-PC characterization studies. We propose a modification of the method to detect and quantify unbound PC of mercury in plant extracts via HPLC coupled to electrospray tandem mass spectrometry (ESI MS-MS) and inductively coupled plasma mass spectrometry (ICP-MS) in parallel. We separated the PC from the mercury by adding the chelating agent sodium 2,3-dimercaptopropanesulfonate monohydrate (DMPS). We only detected the presence of PC after the addition of the chelating agent. Some multicomplexes mPC-nHg could be formed but, due to their large sizes they could not be detected. In the present study, only PC2 was observed in plant samples. Hg accumulation was correlated with PC2 concentration (r2=0.98).






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