Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Published on August 4, 2006; 10.1104/pp.106.085506

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Received June 20, 2006
Accepted July 28, 2006

Dehydroascorbate Reductase Affects Leaf Growth, Development, and Function

Zhong Chen and Daniel R. Gallie *

Department of Biochemistry, University of California, Riverside, CA 92521-0129

* Corresponding author; email: drgallie{at}citrus.ucr.edu.

Ascorbic acid (Asc) is a major antioxidant in plants that detoxifies reactive oxygen species (ROS) and maintains photosynthetic functioning. Expression of dehydroascorbate reductase (DHAR), responsible for regenerating Asc from an oxidized state, regulates the cellular Asc redox state, which in turn, affects cell responsiveness and tolerance to environmental ROS. Because of its role in Asc recycling, we examined whether DHAR is important for plant growth. Suppression of DHAR expression resulted in a preferential loss of chlorophyll a, a lower steady state of ribulose bisphosphate carboxylase/oxygenase as measured by the amount of the large subunit (RbcL), and a lower rate of CO2 assimilation. As a consequence, a slower rate of leaf expansion and reduced foliar dry weight was observed. In addition, an accelerated rate of loss of chlorophyll, RbcL, light-harvesting complex II (LHCII), and photosynthetic functioning was observed in mature leaves resulting in premature leaf aging. Reduced growth rate as measured by plant height and leaf number was consistent with the DHAR-mediated reduction of photosynthetic functioning. Increasing DHAR expression maintained higher levels of chlorophyll, RbcL, LHCII, and photosynthetic functioning resulting in delayed leaf aging. The effect of DHAR expression on leaf aging inversely correlated with the level of lipid peroxidation indicating that DHAR functions to protect against ROS-mediated damage. These observations support the conclusion that through its Asc recycling function, DHAR affects the level of foliar ROS and photosynthetic activity during leaf development and as a consequence, influences the rate of plant growth and leaf aging.




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