Plant Physiology Preview
Published on August 11, 2006; 10.1104/pp.106.085878
OPEN ACCESS ARTICLE
Received June 27, 2006
Accepted August 1, 2006
Dual temporal Role of Plastid Sigma Factor 6 in Arabidopsis Development
Heike Loschelder , Jennifer Schweer , Brigitte Link , and Gerhard Link *
Plant Cell Physiology, University of Bochum, D-44780 Bochum, Germany
* Corresponding author; email: Gerhard.Link{at}ruhr-uni-bochum.de.
Plants contain nuclear-coded sigma factors for initiation of chloroplast transcription. The in vivo function of individual members of the sigma gene family has become increasingly accessible by knockout and complementation strategies. Here we have investigated plastid gene expression in an Arabidopsis thaliana mutant with a defective gene for sigma factor 6. RNA gel blot hybridization and real-time RT-PCR together indicate that this factor has a dual developmental role, with both "early" and "persistent" (long-term) activities. The early role is evident from the sharp decrease of certain plastid transcripts only in young mutant seedlings. The second (persistent) role is reflected by the up- and down-regulation of other transcripts at the time of primary leaf formation and subsequent vegetative development. We conclude that sigma 6 does not represent a general factor but seems to have specialized roles in developmental stage- and gene-specific plastid transcription. The possibility that plastid DNA copy number might be responsible for the altered transcript patterns in mutant vs. wildtype was excluded by the results of DNA gel blot hybridization. Re-transformation of the knockout line with the full-length sigma 6 cDNA further established a causal relationship between the functional sigma gene and the resulting phenotype.
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