Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 27, 2006; 10.1104/pp.106.086363

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
142/4/1480    most recent
pp.106.086363v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Drakakaki, G.
Right arrow Articles by Raikhel, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drakakaki, G.
Right arrow Articles by Raikhel, N.
Agricola
Right arrow Articles by Drakakaki, G.
Right arrow Articles by Raikhel, N.

Received July 5, 2006
Accepted October 17, 2006

Arabidopsis RGP1 and RGP2 are Essential for Pollen Development

Georgia Drakakaki , Olga Zabotina , Ivan Delgado , Stéphanie Robert , Kenneth Keegstra , and Natasha Raikhel *

The Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521
Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan, 48824

* Corresponding author; email: nraikhel{at}ucr.edu.

Reversibly Glycosylated Polypeptides (RGPs) have been implicated in polysaccharide biosynthesis. To date, no direct evidence exists for the involvement of RGPs in a particular biochemical process. The Arabidopsis genome contains five RGP genes out of which RGP1 and RGP2 share the highest sequence identity. We characterized the native expression pattern of Arabidopsis RGPs 1 and 2 and used reverse genetics to investigate their respective functions. Although both genes are ubiquitously expressed, the highest levels are observed in actively growing tissues, and in mature pollen in particular. RGPs showed cytoplasmic and transient association with Golgi. In addition both proteins co-localized in the same compartments and co-immunoprecipitated from plant cell extracts. Single gene disruptions did not show any obvious morphological defects under greenhouse conditions, whereas the double-insertion mutant could not be recovered. We present evidence that the double mutant is lethal, and demonstrate the critical role of RGPs, particularly in pollen development. Detailed analysis demonstrated, that mutant pollen development is associated with abnormally enlarged vacuoles and poorly defined inner cell wall layer, which consequently results in disintegration of the pollen structure during pollen mitosis I. Taken together, our results indicate that RGP1 and RGP2 are required during microspore development and pollen mitosis, either affecting cell division and/or vacuolar integrity.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Persson, A. Paredez, A. Carroll, H. Palsdottir, M. Doblin, P. Poindexter, N. Khitrov, M. Auer, and C. R. Somerville
Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis
PNAS, September 25, 2007; 104(39): 15566 - 15571.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
T. Konishi, T. Takeda, Y. Miyazaki, M. Ohnishi-Kameyama, T. Hayashi, M. A. O'Neill, and T. Ishii
A plant mutase that interconverts UDP-arabinofuranose and UDP-arabinopyranose
Glycobiology, March 1, 2007; 17(3): 345 - 354.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists