Plant Physiol. Bio-Rad Microplate Reader
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 6, 2006; 10.1104/pp.106.087452

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow Supplemental Data
Right arrow All Versions of this Article:
142/4/1493    most recent
pp.106.087452v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chibani, K.
Right arrow Articles by Grappin, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chibani, K.
Right arrow Articles by Grappin, P.
Agricola
Right arrow Articles by Chibani, K.
Right arrow Articles by Grappin, P.

Received July 27, 2006
Accepted September 29, 2006

Proteomic Analysis of Seed Dormancy in Arabidopsis

Kamel Chibani , Sonia Ali-Rachedi , Claudette Job , Dominique Job , Marc Jullien , and Philippe Grappin *

Institut National de la Recherche Agronomique-Institut National Agronomique Paris-Grignon (Etablissement Agro Paris Tech), Chaire de Physiologie Végétale, Unité Mixte de Recherche 204, F-75231 Paris cedex 05, France
Centre National de la Recherche Scientifique-Bayer CropScience Joint Laboratory, Unité Mixte de Recherche 2847, F-69263 Lyon cedex 09, France
Institut National de la Recherche Agronomique-Institut National Agronomique Paris-Grignon (Etablissement Agro Paris Tech), Chaire de Physiologie Végétale, Unité Mixte de Recherche 204, F-75231 Paris cedex 05, France; Institut National de la Recherche Agronomique-Institut National Agronomique Paris-Grignon, Unité Mixte de Recherche 204, F-78026 Versailles cedex, France

* Corresponding author; email: grappin{at}versailles.inra.fr.

The mechanisms controlling seed dormancy in Arabidopsis thaliana have been characterized by proteomics using the dormant accession Cvi originating from the Cape Verde Islands. Comparative studies carried out with freshly harvested dormant (D) and after-ripened non-dormant (ND) seeds revealed a specific differential accumulation of 32 proteins. The data suggested that proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry state leading to dormancy release. Exogenous application of ABA to ND seeds strongly impeded their germination, which physiologically mimicked the behavior of D imbibed seeds. This application resulted in an alteration of the accumulation pattern of 71 proteins. There was a strong down-accumulation of a major part (90%) of these proteins, which were involved mainly in energetic and protein metabolisms. This feature suggested that exogenous ABA triggers proteolytic mechanisms in imbibed seeds. An analysis of de novo protein synthesis by two-dimensional (2D) gel electrophoresis in the presence of [35S]-Met disclosed that exogenous ABA does not impede protein biosynthesis during imbibition. Furthermore, imbibed D seeds proved competent for de novo protein synthesis, demonstrating that impediment of protein translation was not the cause of the observed block of seed germination. However, the 2D protein profiles were markedly different from those obtained with the ND seeds imbibed in ABA. Altogether, the data showed that the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal medium.




This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Gianinetti and P. Vernieri
On the role of abscisic acid in seed dormancy of red rice
J. Exp. Bot., September 26, 2007; (2007) erm198v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Liu, M. Koornneef, and W. J.J. Soppe
The Absence of Histone H2B Monoubiquitination in the Arabidopsis hub1 (rdo4) Mutant Reveals a Role for Chromatin Remodeling in Seed Dormancy
PLANT CELL, February 1, 2007; 19(2): 433 - 444.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists