Plant Physiol. Drug Metab Dispos
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Published on October 13, 2006; 10.1104/pp.106.087700

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Received August 1, 2006
Accepted October 5, 2006

Interactions between MUR10/CesA7 Dependent Secondary Cellulose Biosynthesis and Primary Cell Wall Structure

Sonia Bosca , Christopher J. Barton , Neil G. Taylor , Peter Ryden , Lutz Neumetzler , Markus Pauly , Keith Roberts , and Georg J. Seifert *

John Innes Centre, Norwich Research Park, Colney Norwich, NR4 7UH, UK
Dept Biochemistry, University of Cambridge, Tennis Court Rd, Cambridge CB2 1QW, UK
Centre for Novel Agricultural Products, Department of Biology, University of York, York, YO10 5DD, UK
Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK
Max- Planck Institute for Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany

* Corresponding author; email: georg.seifert{at}boku.ac.at.

Primary cell walls are deposited and remodelled during cell division and expansion. Secondary cell walls are deposited in specialized cells after the expansion phase. It is presently unknown if and how these processes are interrelated. The Arabidopsis thaliana MUR10 gene is required for normal primary cell wall carbohydrate composition in mature leaves as well as for normal plant growth, hypocotyl strength and fertility. The overall sugar composition of young mur10 seedlings is not significantly altered, however the relative proportion of pectin side chains is shifted towards an increase in 1->5-{alpha}-arabinan relative to 1->4-{beta}-galactan. mur10 seedlings display reduced fucogalactosylation of tightly cell wall bound xyloglucan. Expression levels of genes encoding either nucleotide sugar interconversion enzymes or glycosyl transferases, known to be involved in primary and secondary cell wall biosynthesis are generally unaffected, however the CesA7 transcript is specifically suppressed in the mur10-1 allele. The MUR10 locus is identical with the CesA7 gene, which encodes a cellulose catalytic subunit previously thought to be specifically involved in secondary cell wall formation. The xylem vessels in young mur10 hypocotyls are collapsed and their birefringence is lost. Moreover, a fucogalactosylated xyloglucan epitope is reduced and 1->5-{alpha}-arabinan epitope increased in every cell type in mur10 hypocotyls, including cells that do not deposit secondary walls. mur10 also displays an altered distribution of an arabinogalactan-protein (AGP) epitope previously associated with xylem differentiation and secondary wall thickening. This work indicates the existence of a mechanism that senses secondary cell wall integrity and controls biosynthesis or structural remodelling of primary cell walls and cellular differentiation.






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