Plant Physiology Preview
Published on October 13, 2006; 10.1104/pp.106.088682
Received August 23, 2006
Accepted October 10, 2006
A Eukaryotic Factor Required for Accumulation of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis
Ryohei Muraoka , Kenji Okuda , Yoshichika Kobayashi , and Toshiharu Shikanai *
Graduate School of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashiku, Fukuoka, Japan 812-8581
* Corresponding author; email: shikanai{at}agr.kyushu-u.ac.jp.
The NAD(P)H dehydrogenase (NDH) complex in chloroplasts mediates photosystem I cyclic and chlororespiratory electron transport. Eleven chloroplast genes and three nuclear genes have been identified as encoding the Ndh subunits, but the entire subunit composition is still unknown. An Arabidopsis thaliana crr3 (chlororespiratory reduction) mutant was isolated based on its lack of transient increase in chlorophyll fluorescence after actinic light illumination; this was due to a specific defect in accumulation of the NDH complex. The CRR3 gene (At2g01590) encodes a novel protein containing a putative plastid targeting signal and a transmembrane domain. Consistent with the gene structure, CRR3 localized to the membrane fraction of chloroplasts. In addition to the essential function of CRR3 in stabilizing the NDH complex, the NDH complex is also required for the accumulation of CRR3. These results suggest that CRR3 interacts with the NDH complex in the thylakoid membrane. In contrast to other subunits in the chloroplast NDH complex, CRR3 is not conserved in cyanobacteria, from which the chloroplast NDH complex is believed to have originated. We propose that CRR3 is a subunit of the NDH complex, which is specific to the chloroplast.
This article has been cited by other articles:
|
|
|
|
 
Y. N. Munekage, B. Genty, and G. Peltier
Effect of PGR5 Impairment on Photosynthesis and Growth in Arabidopsis thaliana
Plant Cell Physiol.,
November 1, 2008;
49(11):
1688 - 1698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Majeran, B. Zybailov, A. J. Ytterberg, J. Dunsmore, Q. Sun, and K. J. van Wijk
Consequences of C4 Differentiation for Chloroplast Membrane Proteomes in Maize Mesophyll and Bundle Sheath Cells
Mol. Cell. Proteomics,
September 1, 2008;
7(9):
1609 - 1638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mutwil, J. Obro, W. G. T. Willats, and S. Persson
GeneCAT--novel webtools that combine BLAST and co-expression analyses
Nucleic Acids Res.,
July 1, 2008;
36(suppl_2):
W320 - W326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ishikawa, A. Takabayashi, S. Ishida, Y. Hano, T. Endo, and F. Sato
NDF6: A Thylakoid Protein Specific to Terrestrial Plants is Essential for Activity of Chloroplastic NAD(P)H Dehydrogenase in Arabidopsis
Plant Cell Physiol.,
July 1, 2008;
49(7):
1066 - 1073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shimizu, L. Peng, F. Myouga, R. Motohashi, K. Shinozaki, and T. Shikanai
CRR23/NdhL is a Subunit of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis
Plant Cell Physiol.,
May 1, 2008;
49(5):
835 - 842.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Wang and A. R. Portis Jr.
A Novel Nucleus-Encoded Chloroplast Protein, PIFI, Is Involved in NAD(P)H Dehydrogenase Complex-Mediated Chlororespiratory Electron Transport in Arabidopsis
Plant Physiology,
August 1, 2007;
144(4):
1742 - 1752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
