Plant Physiology Preview
Published on October 6, 2006; 10.1104/pp.106.088781
Received August 24, 2006
Accepted September 27, 2006
Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata. VII. Changes in the Plant's proteomes
Ashok P. Giri , Hendrik Wünsche , Sirsha Mitra , Jorge A. Zavala , Alexander Muck , Ale Svato , and Ian T. Baldwin *
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany; Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune-411 008 (M.S.), India
Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany
Mass Spectrometry Research Group, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany
* Corresponding author; email: Baldwin{at}ice.mpg.de.
When Manduca sexta attacks Nicotiana attenuata, fatty acid amino-acid conjugates (FACs) in the larvae's oral secretions (OS) are introduced into feeding wounds. These FACs trigger a transcriptional response that is similar to the response induced by insect damage. Using 2-DE, MALDI-TOF, and LC-MS/MS, we characterized the proteins in phenolic extracts and in a nuclear fraction of leaves elicited by larval attack, and/or in leaves wounded and treated with OS, FAC-free OS, and synthetic FACs. Phenolic extracts yielded  600 protein spots, many of which were altered by elicitation, whereas nuclear protein fractions yielded 100 spots, most of which were unchanged by elicitation. Reproducible elicitor-induced changes in 90 spots were characterized. In general, proteins that increased were involved in primary metabolism, defense, transcriptional and translational regulation; those that decreased were involved in photosynthesis. Like the transcriptional defense responses, proteomic changes were strongly elicited by the FACs in OS. A semi-quantitative RT-PCR approach based on peptide sequences was used to compare transcript and protein accumulation patterns for 17 candidate proteins. In 6 cases the patterns of elicited transcript accumulation were consistent with those of elicited protein accumulation. Functional analysis of one of the identified proteins involved in photosynthesis, RuBPCase activase (RCA), was accomplished by virus-induced gene silencing. Plants with decreased levels of RCA protein had reduced photosynthetic rates and RuBPCase activity, and less biomass, responses consistent with those of herbivore-attacked plants. We conclude that the response of the plant's proteome to herbivore elicitation is complex, and integrated transcriptome-proteome-metabolome analysis is required to fully understand this ubiquitous ecological interaction.
This article has been cited by other articles:
|
|
|
|
 
E. Korner, C. C. von Dahl, G. Bonaventure, and I. T. Baldwin
Pectin methylesterase NaPME1 contributes to the emission of methanol during insect herbivory and to the elicitation of defence responses in Nicotiana attenuata
J. Exp. Bot.,
July 1, 2009;
60(9):
2631 - 2640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Diezel, C. C. von Dahl, E. Gaquerel, and I. T. Baldwin
Different Lepidopteran Elicitors Account for Cross-Talk in Herbivory-Induced Phytohormone Signaling
Plant Physiology,
July 1, 2009;
150(3):
1576 - 1586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Gaquerel, A. Weinhold, and I. T. Baldwin
Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphigidae) and Its Natural Host Nicotiana attenuata. VIII. An Unbiased GCxGC-ToFMS Analysis of the Plant's Elicited Volatile Emissions
Plant Physiology,
March 1, 2009;
149(3):
1408 - 1423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D. Nabity, J. A. Zavala, and E. H. DeLucia
Indirect suppression of photosynthesis on individual leaves by arthropod herbivory
Ann. Bot.,
February 1, 2009;
103(4):
655 - 663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mitra and I. T. Baldwin
Independently Silencing Two Photosynthetic Proteins in Nicotiana attenuata Has Different Effects on Herbivore Resistance
Plant Physiology,
October 1, 2008;
148(2):
1128 - 1138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Pandey, E. Gaquerel, K. Gase, and I. T. Baldwin
RNA-Directed RNA Polymerase3 from Nicotiana attenuata Is Required for Competitive Growth in Natural Environments
Plant Physiology,
July 1, 2008;
147(3):
1212 - 1224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Singh, I. K. Singh, and P. K. Verma
Differential transcript accumulation in Cicer arietinum L. in response to a chewing insect Helicoverpa armigera and defence regulators correlate with reduced insect performance
J. Exp. Bot.,
June 1, 2008;
59(9):
2379 - 2392.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Prins, P. D.R. van Heerden, E. Olmos, K. J. Kunert, and C. H. Foyer
Cysteine proteinases regulate chloroplast protein content and composition in tobacco leaves: a model for dynamic interactions with ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) vesicular bodies
J. Exp. Bot.,
May 1, 2008;
59(7):
1935 - 1950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Pandey, P. Shahi, K. Gase, and I. T. Baldwin
Chemical Ecology Special Feature: Herbivory-induced changes in the small-RNA transcriptome and phytohormone signaling in Nicotiana attenuata
PNAS,
March 25, 2008;
105(12):
4559 - 4564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Zhu-Salzman, D. S. Luthe, and G. W. Felton
Arthropod-Inducible Proteins: Broad Spectrum Defenses against Multiple Herbivores
Plant Physiology,
March 1, 2008;
146(3):
852 - 858.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. S. Chung, A. J.K. Koo, X. Gao, S. Jayanty, B. Thines, A. D. Jones, and G. A. Howe
Regulation and Function of Arabidopsis JASMONATE ZIM-Domain Genes in Response to Wounding and Herbivory
Plant Physiology,
March 1, 2008;
146(3):
952 - 964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. H. Anh, R. Ullrich, D. Benndorf, A. Svatos, A. Muck, and M. Hofrichter
The Coprophilous Mushroom Coprinus radians Secretes a Haloperoxidase That Catalyzes Aromatic Peroxygenation
Appl. Envir. Microbiol.,
September 1, 2007;
73(17):
5477 - 5485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Barazani, C. C. von Dahl, and I. T. Baldwin
Sebacina vermifera Promotes the Growth and Fitness of Nicotiana attenuata by Inhibiting Ethylene Signaling
Plant Physiology,
June 1, 2007;
144(2):
1223 - 1232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chen, E. Gonzales-Vigil, C. G. Wilkerson, and G. A. Howe
Stability of Plant Defense Proteins in the Gut of Insect Herbivores
Plant Physiology,
April 1, 2007;
143(4):
1954 - 1967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
