Plant Physiology Preview
Published on November 10, 2006; 10.1104/pp.106.089003
Received August 29, 2006
Accepted November 1, 2006
Arabidopsis Sucrose Transporter AtSUC9. High Affinity Transport Activity, Intragenic Control of Expression and Early-flowering Mutant Phenotype
Alicia B. Sivitz , Anke Reinders , Meghan E. Johnson , Anthony D. Krentz , Christopher P. L. Grof , Jai M. Perroux , and John M. Ward *
Department of Plant Biology, University of Minnesota Twin Cities, 1445 Gortner Ave., 250 Biological Sciences Center, St. Paul, MN 55108
CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, QLD, 4067 St. Lucia, Australia; Cooperative Research Centre for Sugar Industry Innovation through Biotechnology, The University of Queensland, St Lucia, QLD, 4072
* Corresponding author; email: jward{at}tc.umn.edu.
AtSUC9 (At5g06170), a sucrose transporter from Arabidopsis thaliana (L. Heynh.), was expressed in Xenopus oocytes and transport activity was analyzed. Compared to all other sucrose transporters, AtSUC9 had an ultra-high affinity for sucrose (K0.5 = 0.066 ± 0.025 mM). AtSUC9 showed low substrate specificity, similar to AtSUC2 (At1g22710), and transported a wide range of glucosides including helicin, salicin, arbutin, maltose, fraxin, esculin, turanose, and  -methyl D glucose. The ability of AtSUC9 to transport ten glucosides was compared directly with that of AtSUC2, HvSUT1 (from barley) and ShSUT1 (from sugarcane) and results indicate that Type I and Type II sucrose transporters have different substrate specificities. AtSUC9 protein was localized to the plasma membrane by transient expression in onion epidermis. Using a whole-gene translational fusion to GUS, AtSUC9 expression was found in sink tissues throughout the shoots and in flowers. AtSUC9 expression in Arabidopsis was dependent on intragenic sequence and this was found to also be the true for AtSUC1 (At1g71880) but not AtSUC2. Plants containing mutations in sucrose transporter gene AtSUC9 were found to have an early-flowering phenotype under short-day conditions. The transport properties of AtSUC9 indicate that it is uniquely suited to provide cellular uptake of sucrose at very low extracellular sucrose concentrations. The mutant phenotype of atsuc9 alleles indicates that AtSUC9 activity leads to a delay in floral transition.
This article has been cited by other articles:
|
|
|
|
 
A. C. Srivastava, K. Dasgupta, E. Ajieren, G. Costilla, R. C. McGarry, and B. G. Ayre
Arabidopsis plants harbouring a mutation in AtSUC2, encoding the predominant sucrose/proton symporter necessary for efficient phloem transport, are able to complete their life cycle and produce viable seed
Ann. Bot.,
November 1, 2009;
104(6):
1121 - 1128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rocher, J.-F. Chollet, S. Legros, C. Jousse, R. Lemoine, M. Faucher, D. R. Bush, and J.-L. Bonnemain
Salicylic Acid Transport in Ricinus communis Involves a pH-Dependent Carrier System in Addition to Diffusion
Plant Physiology,
August 1, 2009;
150(4):
2081 - 2091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Berthier, M. Desclos, V. Amiard, A. Morvan-Bertrand, B. Demmig-Adams, W. W. Adams III, R. Turgeon, M.-P. Prud'homme, and N. Noiraud-Romy
Activation of Sucrose Transport in Defoliated Lolium perenne L.: An Example of Apoplastic Phloem Loading Plasticity
Plant Cell Physiol.,
July 1, 2009;
50(7):
1329 - 1344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Braun and T. L. Slewinski
Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading
Plant Physiology,
January 1, 2009;
149(1):
71 - 81.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Morris, H. Tian, S. Park, C. S. Sreevidya, J. M. Ward, and K. D. Hirschi
AtCCX3 Is an Arabidopsis Endomembrane H+-Dependent K+ Transporter
Plant Physiology,
November 1, 2008;
148(3):
1474 - 1486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Srivastava, S. Ganesan, I. O. Ismail, and B. G. Ayre
Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport
Plant Physiology,
September 1, 2008;
148(1):
200 - 211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2007
J. Exp. Bot.,
July 18, 2008;
(2008)
ern109v1.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. B. Sivitz, A. Reinders, and J. M. Ward
Arabidopsis Sucrose Transporter AtSUC1 Is Important for Pollen Germination and Sucrose-Induced Anthocyanin Accumulation
Plant Physiology,
May 1, 2008;
147(1):
92 - 100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. N. Scofield, T. Hirose, N. Aoki, and R. T. Furbank
Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice
J. Exp. Bot.,
September 1, 2007;
58(12):
3155 - 3169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
