Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on October 13, 2006; 10.1104/pp.106.089169

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
142/4/1427    most recent
pp.106.089169v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (13)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pommerrenig, B.
Right arrow Articles by Sauer, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pommerrenig, B.
Right arrow Articles by Sauer, N.
Agricola
Right arrow Articles by Pommerrenig, B.
Right arrow Articles by Sauer, N.

Received August 31, 2006
Accepted October 2, 2006

Plantago major, a Collection of Expressed Sequence Tags from Vascular Tissue and a Simple and Efficient Transformation Method

Benjamin Pommerrenig , Inga Barth , Matthias Niedermeier , Sina Kopp , Jürg Schmid , Rex A. Dwyer , Racella J. McNair , Franz Klebl , and Norbert Sauer *

Molekulare Pflanzenphysiologie, FAU Erlangen-Nürnberg, Staudtstraße 5, D-91058 Erlangen, Germany
Syngenta Biotechnology, Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709, USA

* Corresponding author; email: nsauer{at}biologie.uni-erlangen.de.

The vascular tissue of higher plants consists of specialized cells that differ from all other cells with respect to their shape and size, their organellar composition, their extracellular matrix, the type of their plasmodesmata, and their physiological functions. Intact and pure vascular tissue can be isolated easily and rapidly from leaf blades of Plantago major L. (common plantain), a plant that has been used repeatedly for molecular studies of phloem transport. Here we present a transcriptome analysis based on 5,900 expressed sequence tags (ESTs) and 3,247 independent mRNAs from the Plantago vasculature. The vascular-specificity of these ESTs was confirmed by the identification of well-known phloem or xylem marker genes. Moreover, RT-PCR, macroarray and Northern analyses revealed genes and metabolic pathways that had previously not been described to be vascular-specific. Moreover, Plantago major transformation was established and used to confirm the vascular specificity of a Plantago promoter/{beta}-glucuronidase (GUS) construct in transgenic Plantago plants. Eventually, the applicability and usefulness of the obtained data was demonstrated also for other plant species. Reporter gene constructs generated with promoters from Arabidopsis homologs of newly identified Plantago vascular ESTs revealed vascular specificity of these genes also in Arabidopsis. The presented vascular ESTs and the newly developed transformation system represent an important tool for future studies of functional genomics in the Plantago major vasculature.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
V. V. Radchuk, L. Borisjuk, N. Sreenivasulu, K. Merx, H.-P. Mock, H. Rolletschek, U. Wobus, and W. Weschke
Spatiotemporal Profiling of Starch Biosynthesis and Degradation in the Developing Barley Grain
Plant Physiology, May 1, 2009; 150(1): 190 - 204.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Omid, T. Keilin, A. Glass, D. Leshkowitz, and S. Wolf
Characterization of phloem-sap transcription profile in melon plants
J. Exp. Bot., October 10, 2007; (2007) erm214v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Pommerrenig, F. S. Papini-Terzi, and N. Sauer
Differential Regulation of Sorbitol and Sucrose Loading into the Phloem of Plantago major in Response to Salt Stress
Plant Physiology, June 1, 2007; 144(2): 1029 - 1038.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Malone, Z.-H. Chen, A. R. Bahrami, R. P. Walker, J. E. Gray, and R. C. Leegood
Phosphoenolpyruvate Carboxykinase in Arabidopsis: Changes in Gene Expression, Protein and Activity during Vegetative and Reproductive Development
Plant Cell Physiol., March 1, 2007; 48(3): 441 - 450.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists