Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Plant Physiology Preview
Published on November 10, 2006; 10.1104/pp.106.089524

This Article
Right arrow Full Text (Plant Physiology Preview (PDF))
Right arrow All Versions of this Article:
143/1/300    most recent
pp.106.089524v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Traverso, J. A.
Right arrow Articles by Chueca, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Traverso, J. A.
Right arrow Articles by Chueca, A.
Agricola
Right arrow Articles by Traverso, J. A.
Right arrow Articles by Chueca, A.

Received September 6, 2006
Accepted November 6, 2006

PsTRXh1 and PsTRXh2, are both Pea (Pisum sativum) h-type Thioredoxins with Antagonistic Behaviour in Redox Imbalances

José A. Traverso *, Florence Vignols , Roland Cazalis , Amada Pulido , Mariam Sahrawy , Francisco Javier Cejudo , Yves Meyer , and Ana Chueca

Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), C/ Prof. Albareda 1, 18008-Granada, Spain
Laboratoire Génome et Développement des Plantes, Université de Perpignan, UMR 5096 CNRS-UP-IRD, 66860 Perpignan, France
Laboratoire d'Agrophysiologie, UMR 1054 INRA, ESA Purpan, 31076 Toulouse Cedex 3, France
Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Avda. Américo Vespucio 49, 41092-Sevilla, Spain

* Corresponding author; email: jose.traverso{at}isv.cnrs-gif.fr.

Thioredoxins (TRXs) are small ubiquitous oxidoreductases involved in disulfide bond reduction of a large panel of target proteins. The most complex cluster in the family of plant thioredoxins is formed by h-type TRXs. In Arabidopsis thaliana, nine members of this subgroup were described, which are less well known than their plastidial counterparts. The functional study of TRXhs is difficult because of the high number of isoforms and their similar biochemical characteristics, thus arising the question whether they have specific or redundant functions. Type-h TRXs are involved in seed germination and self-incompatibility in pollen-pistil interaction. Their function as antioxidants has recently been proposed but further work is needed to clarify this function in plants. In this study, we describe two new h-type TRXs from Pisum sativum (stated PsTRXh1 and PsTRXh2). By functional complementation of a yeast trx1{Delta} trx2{Delta} double mutant, we demonstrate that PsTRXh1 is involved in the redox-imbalance control, possibly through its interaction with peroxiredoxins. In contrast, PsTRXh2 provokes a phenotype of hypersensitivity to hydrogen peroxide in the yeast mutant. Furthermore, we show differential gene expression and protein accumulation of the two isoforms, PsTRXh1 protein being abundantly detected in vascular tissue and flowers, whereas PsTRXh2 gene expression was hardly detectable. By comparison with previous data of additional PsTRXh isoforms, our results indicate specific functions for the pea h-type thioredoxins so far described.




This article has been cited by other articles:


Home page
 J Exp BotHome page
J. A. Traverso, F. Vignols, R. Cazalis, A. J. Serrato, P. Pulido, M. Sahrawy, Y. Meyer, F. J. Cejudo, and A. Chueca
Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues
J. Exp. Bot., April 1, 2008; 59(6): 1267 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. de Dios Barajas-Lopez, A. J. Serrato, A. Olmedilla, A. Chueca, and M. Sahrawy
Localization in Roots and Flowers of Pea Chloroplastic Thioredoxin f and Thioredoxin m Proteins Reveals New Roles in Nonphotosynthetic Organs
Plant Physiology, November 1, 2007; 145(3): 946 - 960.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists